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-Structure paper
タイトル | Structure of the PCNA unloader Elg1-RFC. |
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ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 9, Page eadl1739, Year 2024 |
掲載日 | 2024年3月1日 |
著者 | Fengwei Zheng / Nina Y Yao / Roxana E Georgescu / Huilin Li / Michael E O'Donnell / |
PubMed 要旨 | During DNA replication, the proliferating cell nuclear antigen (PCNA) clamps are loaded onto primed sites for each Okazaki fragment synthesis by the AAA heteropentamer replication factor C (RFC). ...During DNA replication, the proliferating cell nuclear antigen (PCNA) clamps are loaded onto primed sites for each Okazaki fragment synthesis by the AAA heteropentamer replication factor C (RFC). PCNA encircling duplex DNA is quite stable and is removed from DNA by the dedicated clamp unloader Elg1-RFC. Here, we show the cryo-EM structure of Elg1-RFC in various states with PCNA. The structures reveal essential features of Elg1-RFC that explain how it is dedicated to PCNA unloading. Specifically, Elg1 contains two external loops that block opening of the Elg1-RFC complex for DNA binding, and an "Elg1 plug" domain that fills the central DNA binding chamber, thereby reinforcing the exclusive PCNA unloading activity of Elg1-RFC. Elg1-RFC was capable of unloading PCNA using non-hydrolyzable AMP-PNP. Both RFC and Elg1-RFC could remove PCNA from covalently closed circular DNA, indicating that PCNA unloading occurs by a mechanism that is distinct from PCNA loading. Implications for the PCNA unloading mechanism are discussed. |
リンク | Sci Adv / PubMed:38427736 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.2 - 3.67 Å |
構造データ | EMDB-41252: Cryo-EM map of the Saccharomyces cerevisiae PCNA clamp unloader Elg1-RFC complex EMDB-41253: Cryo-EM map of the Saccharomyces cerevisiae clamp unloader Elg1-RFC bound to a cracked PCNA EMDB-41254: Cryo-EM map of the Saccharomyces cerevisiae clamp unloader Elg1-RFC bound to PCNA |
化合物 | ChemComp-AGS: ChemComp-MG: ChemComp-ADP: |
由来 |
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キーワード | REPLICATION / DNA replication / DNA sliding clamp / PCNA clamp / clamp unloader / Elg1-RFC unloader / clamp loader/unloader |