EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural dynamics at cytosolic interprotomer interfaces control gating of a mammalian TRPM5 channel.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 121, Issue 27, Page e2403333121, Year 2024
掲載日	2024年7月2日
著者	Sebastian Karuppan / Lynn Goss Schrag / Caroline M Pastrano / Andrés Jara-Oseguera / Lejla Zubcevic /
PubMed 要旨	The transient receptor potential melastatin (TRPM) tetrameric cation channels are involved in a wide range of biological functions, from temperature sensing and taste transduction to regulation of ...The transient receptor potential melastatin (TRPM) tetrameric cation channels are involved in a wide range of biological functions, from temperature sensing and taste transduction to regulation of cardiac function, inflammatory pain, and insulin secretion. The structurally conserved TRPM cytoplasmic domains make up >70 % of the total protein. To investigate the mechanism by which the TRPM cytoplasmic domains contribute to gating, we employed electrophysiology and cryo-EM to study TRPM5-a channel that primarily relies on activation via intracellular Ca. Here, we show that activation of mammalian TRPM5 channels is strongly altered by Ca-dependent desensitization. Structures of rat TRPM5 identify a series of conformational transitions triggered by Ca binding, whereby formation and dissolution of cytoplasmic interprotomer interfaces appear to control activation and desensitization of the channel. This study shows the importance of the cytoplasmic assembly in TRPM5 channel function and sets the stage for future investigations of other members of the TRPM family.
リンク	Proc Natl Acad Sci U S A / PubMed:38923985
手法	EM (単粒子)
解像度	3.5 - 5.0 Å
構造データ	40574 8sl6 EMDB-40574, PDB-8sl6: Cryo-EM structure of the rat TRPM5 channel in EGTA 手法: EM (単粒子) / 解像度: 4.3 Å 40575 8sl8 EMDB-40575, PDB-8sl8: Cryo-EM structure of the rat TRPM5 channel in trace calcium, trace-1 手法: EM (単粒子) / 解像度: 4.2 Å 40576 8sla EMDB-40576, PDB-8sla: Cryo-EM structure of the rat TRPM5 channel in trace calcium, trace-2 手法: EM (単粒子) / 解像度: 3.7 Å 40577 8sle EMDB-40577, PDB-8sle: Cryo-EM structure of the rat TRPM5 channel in trace calcium, trace-3 手法: EM (単粒子) / 解像度: 4.3 Å 40578 8sli EMDB-40578, PDB-8sli: Cryo-EM structure of the rat TRPM5 channel in 2mM calcium, high-1 手法: EM (単粒子) / 解像度: 4.9 Å 40579 8slp EMDB-40579, PDB-8slp: Cryo-EM structure of the rat TRPM5 channel in 2mM calcium, high-2 手法: EM (単粒子) / 解像度: 3.8 Å 40580 8slq EMDB-40580, PDB-8slq: Cryo-EM structure of the rat TRPM5 channel in 2mM calcium, high-3 手法: EM (単粒子) / 解像度: 4.5 Å 40581 8slw EMDB-40581, PDB-8slw: Cryo-EM structure of the rat TRPM5 channel in 2mM calcium, high-4 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-40592: Focused map of the cytoplasmic domains of rat TRPM5, conformation high-4 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-40593: Focused map of the cytoplasmic domains of rat TRPM5, conformation high-3 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-40595: Focused map of the cytoplasmic domains of rat TRPM5, conformation high-2 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-40596: Focused map of the cytoplasmic domains of rat TRPM5, conformation high-1 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-40597: Focused map of the cytoplasmic domains of rat TRPM5, conformation trace-3 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-40599: Focused map of the cytoplasmic domains of rat TRPM5, conformation trace-2 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-40600: Focused map of the cytoplasmic domains of rat TRPM5, conformation trace-1 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	rattus norvegicus (ドブネズミ)
キーワード	MEMBRANE PROTEIN / ion channel / Transient Receptor Potential / TRP / Transient Receptor Potential Melastatin 5 / TRPM5 / TRANSPORT PROTEIN