EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for receptor-binding domain mobility of the spike in SARS-CoV-2 BA.2.86 and JN.1.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 8574, Year 2024
掲載日	2024年10月7日
著者	Hisano Yajima / Yuki Anraku / Yu Kaku / Kanako Terakado Kimura / Arnon Plianchaisuk / Kaho Okumura / Yoshiko Nakada-Nakura / Yusuke Atarashi / Takuya Hemmi / Daisuke Kuroda / Yoshimasa Takahashi / Shunsuke Kita / Jiei Sasaki / Hiromi Sumita / / Jumpei Ito / Katsumi Maenaka / Kei Sato / Takao Hashiguchi /
PubMed 要旨	Since 2019, SARS-CoV-2 has undergone mutations, resulting in pandemic and epidemic waves. The SARS-CoV-2 spike protein, crucial for cellular entry, binds to the ACE2 receptor exclusively when its ...Since 2019, SARS-CoV-2 has undergone mutations, resulting in pandemic and epidemic waves. The SARS-CoV-2 spike protein, crucial for cellular entry, binds to the ACE2 receptor exclusively when its receptor-binding domain (RBD) adopts the up-conformation. However, whether ACE2 also interacts with the RBD in the down-conformation to facilitate the conformational shift to RBD-up remains unclear. Herein, we present the structures of the BA.2.86 and the JN.1 spike proteins bound to ACE2. Notably, we successfully observed the ACE2-bound down-RBD, indicating an intermediate structure before the RBD-up conformation. The wider and mobile angle of RBDs in the up-state provides space for ACE2 to interact with the down-RBD, facilitating the transition to the RBD-up state. The K356T, but not N354-linked glycan, contributes to both of infectivity and neutralizing-antibody evasion in BA.2.86. These structural insights the spike-protein dynamics would help understand the mechanisms underlying SARS-CoV-2 infection and its neutralization.
リンク	Nat Commun / PubMed:39375326 / PubMed Central
手法	EM (単粒子)
解像度	2.59 - 4.3 Å
構造データ	37910 8wxl EMDB-37910, PDB-8wxl: Structure of the SARS-CoV-2 BA.2.86 spike glycoprotein (closed state) 手法: EM (単粒子) / 解像度: 2.59 Å 38459 8xux EMDB-38459, PDB-8xux: Structure of the SARS-CoV-2 BA.2.86 spike protein (1-up state) 手法: EM (単粒子) / 解像度: 3.32 Å 38686 8xuy EMDB-38686, PDB-8xuy: Structure of SARS-CoV-2 BA.2.86 spike glycoprotein in complex with ACE2 (2-up state) 手法: EM (単粒子) / 解像度: 3.14 Å 38687 8xuz EMDB-38687, PDB-8xuz: Structure of SARS-CoV-2 BA.2.86 spike glycoprotein in complex with ACE2 (2-up and 1-down state) 手法: EM (単粒子) / 解像度: 3.05 Å 38688 8xv0 EMDB-38688, PDB-8xv0: Structure of SARS-CoV-2 BA.2.86 spike RBD in complex with ACE2 (up state) 手法: EM (単粒子) / 解像度: 3.0 Å 38689 8xv1 EMDB-38689, PDB-8xv1: Structure of SARS-CoV-2 BA.2.86 spike RBD in complex with ACE2 (down state) 手法: EM (単粒子) / 解像度: 3.05 Å 38690 8xvm EMDB-38690, PDB-8xvm: Structure of SARS-CoV-2 BA.2.86 spike glycoprotein in complex with ACE2 (3-up state) 手法: EM (単粒子) / 解像度: 2.77 Å 60886 9iu1 EMDB-60886, PDB-9iu1: Structure of SARS-CoV-2 JN.1 spike RBD in complex with ACE2 (up state) 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-60904: Structure of SARS-CoV-2 JN.1 spike glycoprotein in complex with ACE2 (2-up state) 手法: EM (単粒子) / 解像度: 3.38 Å EMDB-60905: Structure of SARS-CoV-2 BA.2.86 spike glycoprotein in complex with ACE2 (1 highly-open RBD and 1 partially-open RBD) 手法: EM (単粒子) / 解像度: 4.15 Å EMDB-60906: Structure of SARS-CoV-2 JN.1 spike glycoprotein in complex with ACE2 (2-up and 1-down state) 手法: EM (単粒子) / 解像度: 3.39 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN / spike protein / glycoprotein / VIRUS / VIRAL PROTEIN/PROTEIN BINDING / VIRAL PROTEIN-PROTEIN BINDING complex