EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Correction of preferred orientation-induced distortion in cryo-electron microscopy maps.
ジャーナル・号・ページ	Sci Adv, Vol. 10, Issue 30, Page eadn0092, Year 2024
掲載日	2024年7月26日
著者	Dongjie Zhu / Weili Cao / Junxi Li / Chunling Wu / Duanfang Cao / Xinzheng Zhang /
PubMed 要旨	Reconstruction maps of cryo-electron microscopy (cryo-EM) exhibit distortion when the cryo-EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts had been ...Reconstruction maps of cryo-electron microscopy (cryo-EM) exhibit distortion when the cryo-EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts had been attempted to address this preferred orientation problem using tilt-collection strategy and modifications to grids or to air-water interfaces. However, these approaches often require time-consuming experiments, and the effect was always protein dependent. Here, we developed a procedure containing removing misaligned particles and an iterative reconstruction method based on signal-to-noise ratio of Fourier component to correct this distortion by recovering missing data using a purely computational algorithm. This procedure called signal-to-noise ratio iterative reconstruction method (SIRM) was applied on incomplete datasets of various proteins to fix distortion in cryo-EM maps and to a more isotropic resolution. In addition, SIRM provides a better reference map for further reconstruction refinements, resulting in an improved alignment, which ultimately improves map quality and benefits model building.
リンク	Sci Adv / PubMed:39058771
手法	EM (単粒子)
解像度	3.16 - 3.8 Å
構造データ	EMDB-38080: SIRM reconstruction of the MC-45 de novo processed ribosome 50S 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-38081: Conventional Reconstruction of the MC-45 de novo processed ribosome 50S 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-38082: SIRM reconstruction of the unpublished protein 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-38083: The SIRM reconstruction of the MC-40 de novo processed HA-trimer 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-38084: The conventional reconstruction of the MC-40 de novo processed HA-trimer 手法: EM (単粒子) / 解像度: 3.38 Å EMDB-38085: The SIRM reconstruction of the MC-45 de novo processed PS1 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-38086: The conventional reconstruction of the MC-45 de novo processed PS1 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-39299: Human resource SGLT1-MAP17 complex 手法: EM (単粒子) / 解像度: 3.3 Å
由来	Escherichia coli (大腸菌) Homo sapiens (ヒト) Chlamydomonas reinhardtii (クラミドモナス) santria sp sf9 (ツマジロクサヨトウ)