+検索条件
-Structure paper
タイトル | Correction of preferred orientation-induced distortion in cryo-electron microscopy maps. |
---|---|
ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 30, Page eadn0092, Year 2024 |
掲載日 | 2024年7月26日 |
![]() | Dongjie Zhu / Weili Cao / Junxi Li / Chunling Wu / Duanfang Cao / Xinzheng Zhang / ![]() |
PubMed 要旨 | Reconstruction maps of cryo-electron microscopy (cryo-EM) exhibit distortion when the cryo-EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts had been ...Reconstruction maps of cryo-electron microscopy (cryo-EM) exhibit distortion when the cryo-EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts had been attempted to address this preferred orientation problem using tilt-collection strategy and modifications to grids or to air-water interfaces. However, these approaches often require time-consuming experiments, and the effect was always protein dependent. Here, we developed a procedure containing removing misaligned particles and an iterative reconstruction method based on signal-to-noise ratio of Fourier component to correct this distortion by recovering missing data using a purely computational algorithm. This procedure called signal-to-noise ratio iterative reconstruction method (SIRM) was applied on incomplete datasets of various proteins to fix distortion in cryo-EM maps and to a more isotropic resolution. In addition, SIRM provides a better reference map for further reconstruction refinements, resulting in an improved alignment, which ultimately improves map quality and benefits model building. |
![]() | ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 3.16 - 3.8 Å |
構造データ | ![]() EMDB-38080: SIRM reconstruction of the MC-45 de novo processed ribosome 50S ![]() EMDB-38081: Conventional Reconstruction of the MC-45 de novo processed ribosome 50S ![]() EMDB-38082: SIRM reconstruction of the unpublished protein ![]() EMDB-38083: The SIRM reconstruction of the MC-40 de novo processed HA-trimer ![]() EMDB-38084: The conventional reconstruction of the MC-40 de novo processed HA-trimer ![]() EMDB-38085: The SIRM reconstruction of the MC-45 de novo processed PS1 ![]() EMDB-38086: The conventional reconstruction of the MC-45 de novo processed PS1 ![]() EMDB-39299: Human resource SGLT1-MAP17 complex |
由来 |
|