EMDB/PDB/SASBDBから引用されている文献のデータベース

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構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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IF	>30 >20 >10 >5 all

タイトル	Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 638, Year 2024
掲載日	2024年1月20日
著者	Niraj Kumar / Shivani Sharma / Prem S Kaushal /
PubMed 要旨	Ribosome hibernation is a key survival strategy bacteria adopt under environmental stress, where a protein, hibernation promotion factor (HPF), transitorily inactivates the ribosome. Mycobacterium ...Ribosome hibernation is a key survival strategy bacteria adopt under environmental stress, where a protein, hibernation promotion factor (HPF), transitorily inactivates the ribosome. Mycobacterium tuberculosis encounters hypoxia (low oxygen) as a major stress in the host macrophages, and upregulates the expression of RafH protein, which is crucial for its survival. The RafH, a dual domain HPF, an orthologue of bacterial long HPF (HPF), hibernates ribosome in 70S monosome form, whereas in other bacteria, the HPF induces 70S ribosome dimerization and hibernates its ribosome in 100S disome form. Here, we report the cryo- EM structure of M. smegmatis, a close homolog of M. tuberculosis, 70S ribosome in complex with the RafH factor at an overall 2.8 Å resolution. The N- terminus domain (NTD) of RafH binds to the decoding center, similarly to HPF NTD. In contrast, the C- terminus domain (CTD) of RafH, which is larger than the HPF CTD, binds to a distinct site at the platform binding center of the ribosomal small subunit. The two domain-connecting linker regions, which remain mostly disordered in earlier reported HPF structures, interact mainly with the anti-Shine Dalgarno sequence of the 16S rRNA.
リンク	Nat Commun / PubMed:38245551 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.5 Å
構造データ	37551 8whx EMDB-37551, PDB-8whx: Cryo- EM structure of Mycobacterium smegmatis 70S ribosome and RafH. 手法: EM (単粒子) / 解像度: 2.8 Å 37552 8why EMDB-37552, PDB-8why: Cryo- EM structure of Mycobacterium smegmatis 50S ribosomal subunit (body 1) of 70S ribosome and RafH. 手法: EM (単粒子) / 解像度: 2.7 Å 37559 8wi7 EMDB-37559, PDB-8wi7: Cryo- EM structure of Mycobacterium smegmatis 70S ribosome, bS1 and RafH. 手法: EM (単粒子) / 解像度: 3.5 Å 37560 8wi8 EMDB-37560, PDB-8wi8: Cryo- EM structure of Mycobacterium smegmatis 50S ribosomal subunit (body 1) of 70S ribosome, bS1 and RafH. 手法: EM (単粒子) / 解像度: 2.7 Å 37561 8wi9 EMDB-37561, PDB-8wi9: Cryo- EM structure of Mycobacterium smegmatis 30S ribosomal subunit (body 2) of 70S ribosome, bS1 and RafH. 手法: EM (単粒子) / 解像度: 3.5 Å 37562 8wib EMDB-37562, PDB-8wib: Cryo- EM structure of Mycobacterium smegmatis 70S ribosome, E- tRNA and RafH. 手法: EM (単粒子) / 解像度: 3.5 Å 37563 8wic EMDB-37563, PDB-8wic: Cryo- EM structure of Mycobacterium smegmatis 50S ribosomal subunit (body 1) of 70S ribosome, E- tRNA and RafH. 手法: EM (単粒子) / 解像度: 3.5 Å 37564 8wid EMDB-37564, PDB-8wid: Cryo- EM structure of Mycobacterium smegmatis 30S ribosomal subunit (body 2) of 70S ribosome, E- tRNA and RafH. 手法: EM (単粒子) / 解像度: 3.5 Å 37565 8wif EMDB-37565, PDB-8wif: Cryo- EM structure of Mycobacterium smegmatis 30S ribosomal subunit (body 2) of 70S ribosome and RafH. 手法: EM (単粒子) / 解像度: 2.9 Å
由来	mycolicibacterium smegmatis mc2 155 (バクテリア)
キーワード	RIBOSOME / protein synthesis / Mycobacterium smegmatis / hibernation promotion factor / HPF / RafH / hypoxia stress / Cryo- EM / Single particle reconstruction / Single particle reconstruction.