EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1.
ジャーナル・号・ページ	Science, Vol. 381, Issue 6660, Page eadd8673, Year 2023
掲載日	2023年8月25日
著者	Chao-Pei Liu / Zhenyu Yu / Jun Xiong / Jie Hu / Aoqun Song / Dongbo Ding / Cong Yu / Na Yang / Mingzhu Wang / Juan Yu / Peini Hou / Kangning Zeng / Zhenyu Li / Zhuqiang Zhang / Xinzheng Zhang / Wei Li / Zhiguo Zhang / Bing Zhu / Guohong Li / Rui-Ming Xu /
PubMed 要旨	Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly ...Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication.
リンク	Science / PubMed:37616371 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.42 - 6.6 Å
構造データ	33625 7y5u EMDB-33625, PDB-7y5u: Cryo-EM structure of the monomeric human CAF1LC-H3-H4 complex 手法: EM (単粒子) / 解像度: 3.8 Å 33626 7y5v EMDB-33626, PDB-7y5v: Cryo-EM structure of the dimeric human CAF1LC-H3-H4 complex 手法: EM (単粒子) / 解像度: 6.1 Å 33627 7y5w EMDB-33627, PDB-7y5w: Cryo-EM structure of the left-handed Di-tetrasome 手法: EM (単粒子) / 解像度: 3.5 Å 33630 7y60 EMDB-33630, PDB-7y60: Cryo-EM structure of human CAF1LC bound right-handed Di-tetrasome 手法: EM (単粒子) / 解像度: 3.8 Å 33631 7y61 EMDB-33631, PDB-7y61: Cryo-EM structure of the two CAF1LCs bound right-handed Di-tetrasome 手法: EM (単粒子) / 解像度: 5.6 Å 35660 8iqf EMDB-35660: Composite cryo-EM density map of the dimeric human CAF1-H3-H4 complex PDB-8iqf: Cryo-EM structure of the dimeric human CAF1-H3-H4 complex 手法: EM (単粒子) / 解像度: 4.6 Å 35661 8iqg EMDB-35661, PDB-8iqg: Cryo-EM structure of the monomeric human CAF1-H3-H4 complex 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-35708: Cryo-EM consensus map of the dimeric human CAF1-H3-H4 complex 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-35709: Local refinement cryo-EM map of protomer I of the dimeric human CAF1-H3-H4 complex 手法: EM (単粒子) / 解像度: 4.63 Å EMDB-35710: Local refinement cryo-EM map of protomer II of the dimeric human CAF1-H3-H4 complex 手法: EM (単粒子) / 解像度: 4.58 Å 36013 8j6s EMDB-36013, PDB-8j6s: Cryo-EM structure of the single CAF-1 bound right-handed Di-tetrasome 手法: EM (単粒子) / 解像度: 3.8 Å 36014 8j6t EMDB-36014, PDB-8j6t: Cryo-EM structure of the double CAF-1 bound right-handed Di-tetrasome 手法: EM (単粒子) / 解像度: 6.6 Å PDB-7y5k: Crystal structure of human CAF-1 core complex in spacegroup C2221 手法: X-RAY DIFFRACTION / 解像度: 3.48 Å PDB-7y5l: Crystal structure of human CAF-1 core complex in spacegroup C2 手法: X-RAY DIFFRACTION / 解像度: 3.42 Å PDB-7y5o: Crystal structure of human CAF-1 core complex in spacegroup P21 手法: X-RAY DIFFRACTION / 解像度: 3.57 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-HOH: WATER
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	REPLICATION / Histone chaperone / Chromatin assembly factor / DNA BINDING PROTEIN/DNA / Left-handed / Di-tetrasome / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / REPLICATION/DNA / REPLICATION-DNA COMPLEX