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-Structure paper
タイトル | Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 6476, Year 2022 |
掲載日 | 2022年10月29日 |
著者 | Qianqian Shao / Irina V Agarkova / Eric A Noel / David D Dunigan / Yunshu Liu / Aohan Wang / Mingcheng Guo / Linlin Xie / Xinyue Zhao / Michael G Rossmann / James L Van Etten / Thomas Klose / Qianglin Fang / |
PubMed 要旨 | Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play ...Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play critical roles in the viral life cycles, identities and high-resolution structures of these components remain unknown. Here, by determining a near-atomic-resolution, five-fold averaged structure of Paramecium bursaria chlorella virus 1, we unexpectedly found the viral capsid possesses up to five major capsid protein variants and a penton protein variant. These variants create varied capsid microenvironments for the associations of fibers, a vesicle, and previously unresolved minor capsid proteins. Our structure reveals the identities and atomic models of the capsid components that do not obey the overall icosahedral symmetry and leads to a model for how these components are assembled and initiate capsid assembly, and this model might be applicable to many other giant viruses. |
リンク | Nat Commun / PubMed:36309542 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.8 - 4.9 Å |
構造データ | EMDB-34438, PDB-8h2i: EMDB-34439: Five-fold averaged cryo-EM reconstruction of PBCV-1 before applying the "block-based" reconstruction method EMDB-35326: Cryo-EM reconstruction of PBCV-1 capsid block 1 EMDB-35490: Cryo-EM reconstruction of PBCV-1 capsid block 2 EMDB-35585: Cryo-EM reconstruction of PBCV-1 capsid block 3 EMDB-35586: Cryo-EM reconstruction of PBCV-1 capsid block 4 EMDB-35589: Cryo-EM reconstruction of PBCV-1 capsid block 5 EMDB-35593: Cryo-EM reconstruction of PBCV-1 capsid block 6 EMDB-35594: Cryo-EM reconstruction of PBCV-1 capsid block 8 EMDB-35644: Cryo-EM reconstruction of PBCV-1 capsid block 9 EMDB-35645: Cryo-EM reconstruction of PBCV-1 capsid block 7 EMDB-35646: Cryo-EM reconstruction of PBCV-1 capsid block 10 EMDB-35647: Cryo-EM reconstruction of PBCV-1 capsid block 11 EMDB-35648: Cryo-EM reconstruction of PBCV-1 capsid block 12 EMDB-35650: Cryo-EM reconstruction of PBCV-1 capsid block 13 EMDB-35656: Cryo-EM reconstruction of PBCV-1 capsid block 14 EMDB-35659: Cryo-EM reconstruction of PBCV-1 capsid block 15 EMDB-35695: Cryo-EM reconstruction of PBCV-1 capsid block 16 EMDB-35954: Cryo-EM reconstruction of PBCV-1 capsid block 17 EMDB-35955: Cryo-EM reconstruction of PBCV-1 capsid block 18 EMDB-35956: Cryo-EM reconstruction of PBCV-1 capsid block 19 EMDB-35957: Cryo-EM reconstruction of PBCV-1 capsid block 20 EMDB-35958: Cryo-EM reconstruction of PBCV-1 capsid block 21 EMDB-35959: Cryo-EM reconstruction of PBCV-1 capsid block 22 EMDB-35960: Cryo-EM reconstruction of PBCV-1 capsid block 23 |
由来 |
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キーワード | VIRAL PROTEIN / giant virus / nucleocytoplasmic large DNA viruses (NCLDVs) / viral assembly / Paramecium bursaria chlorella virus 1 (PBCV-1) / chlorovirus / major capsid protein / minor capsid protein / VIRUS |