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- EMDB-34438: Near-atomic structure of five-fold averaged PBCV-1 capsid -

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Basic information

Entry
Database: EMDB / ID: EMD-34438
TitleNear-atomic structure of five-fold averaged PBCV-1 capsid
Map data
Sample
  • Virus: Paramecium bursaria Chlorella virus 1
    • Protein or peptide: x 26 types
Keywordsgiant virus / nucleocytoplasmic large DNA viruses (NCLDVs) / viral assembly / Paramecium bursaria chlorella virus 1 (PBCV-1) / chlorovirus / major capsid protein / minor capsid protein / VIRUS / VIRAL PROTEIN
Function / homology
Function and homology information


viral capsid / membrane => GO:0016020 / structural molecule activity / membrane
Similarity search - Function
Chlorovirus glycoprotein repeat / Domain of unknown function DUF5901 / Chlorovirus glycoprotein repeat / Family of unknown function (DUF5901) / Protein of unknown function DUF5762 / Protein of unknown function DUF5761 / Domain of unknown function DUF5899 / Family of unknown function (DUF5761) / Family of unknown function (DUF5762) / Family of unknown function (DUF5899) ...Chlorovirus glycoprotein repeat / Domain of unknown function DUF5901 / Chlorovirus glycoprotein repeat / Family of unknown function (DUF5901) / Protein of unknown function DUF5762 / Protein of unknown function DUF5761 / Domain of unknown function DUF5899 / Family of unknown function (DUF5761) / Family of unknown function (DUF5762) / Family of unknown function (DUF5899) / Peptidase G2, IMC autoproteolytic cleavage domain / Peptidase_G2, IMC autoproteolytic cleavage domain / Major capsid protein, N-terminal / Major capsid protein N-terminus / Major capsid protein, C-terminal / Major capsid protein, C-terminal domain superfamily / Large eukaryotic DNA virus major capsid protein / Group II dsDNA virus coat/capsid protein / Parallel beta-helix repeat / Parallel beta-helix repeats
Similarity search - Domain/homology
Major capsid protein VP54 / DUF5901 domain-containing protein / Capsid protein / Uncharacterized protein / Capsid protein / Capsid protein / Major capsid protein / Peptidase_G2 domain-containing protein / Uncharacterized protein / PsbP C-terminal domain-containing protein ...Major capsid protein VP54 / DUF5901 domain-containing protein / Capsid protein / Uncharacterized protein / Capsid protein / Capsid protein / Major capsid protein / Peptidase_G2 domain-containing protein / Uncharacterized protein / PsbP C-terminal domain-containing protein / Transmembrane protein / A2M domain-containing protein / Uncharacterized protein / DUF5899 domain-containing protein / Uncharacterized protein / Capsid protein / Capsid protein / Chlorovirus glycoprotein repeat domain-containing protein / Peptidase S74 domain-containing protein / Chlorovirus glycoprotein repeat domain-containing protein / SAYSvFN domain-containing protein / Uncharacterized protein / Glycine-rich protein / Entry/fusion complex component / Capsid protein / Uncharacterized protein
Similarity search - Component
Biological speciesParamecium bursaria Chlorella virus 1
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsShao Q / Agarkova IV / Noel EA / Dunigan DD / Liu Y / Wang A / Guo M / Xie L / Zhao X / Rossmann MG ...Shao Q / Agarkova IV / Noel EA / Dunigan DD / Liu Y / Wang A / Guo M / Xie L / Zhao X / Rossmann MG / Van Etten JL / Klose T / Fang Q
Funding support United States, 2 items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)1736030 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI011219 United States
CitationJournal: Nat Commun / Year: 2022
Title: Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1.
Authors: Qianqian Shao / Irina V Agarkova / Eric A Noel / David D Dunigan / Yunshu Liu / Aohan Wang / Mingcheng Guo / Linlin Xie / Xinyue Zhao / Michael G Rossmann / James L Van Etten / Thomas Klose / Qianglin Fang /
Abstract: Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play ...Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play critical roles in the viral life cycles, identities and high-resolution structures of these components remain unknown. Here, by determining a near-atomic-resolution, five-fold averaged structure of Paramecium bursaria chlorella virus 1, we unexpectedly found the viral capsid possesses up to five major capsid protein variants and a penton protein variant. These variants create varied capsid microenvironments for the associations of fibers, a vesicle, and previously unresolved minor capsid proteins. Our structure reveals the identities and atomic models of the capsid components that do not obey the overall icosahedral symmetry and leads to a model for how these components are assembled and initiate capsid assembly, and this model might be applicable to many other giant viruses.
History
DepositionOct 6, 2022-
Header (metadata) releaseNov 16, 2022-
Map releaseNov 16, 2022-
UpdateSep 6, 2023-
Current statusSep 6, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_34438.png

Downloads & links

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Map

FileDownload / File: emd_34438.map.gz / Format: CCP4 / Size: 6.4 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.62 Å
Density
Contour LevelBy AUTHOR: 0.55
Minimum - Maximum-1.5407423 - 2.269498
Average (Standard dev.)0.0007873746 (±0.09836302)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-600-600-600
Dimensions120012001200
Spacing120012001200
CellA=B=C: 1944.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Paramecium bursaria Chlorella virus 1

EntireName: Paramecium bursaria Chlorella virus 1
Components
  • Virus: Paramecium bursaria Chlorella virus 1
    • Protein or peptide: Major capsid protein (MCP)
    • Protein or peptide: MCPv1
    • Protein or peptide: MCPv2
    • Protein or peptide: MCPv3
    • Protein or peptide: MCPv4
    • Protein or peptide: P1v1
    • Protein or peptide: P2
    • Protein or peptide: P3
    • Protein or peptide: P4
    • Protein or peptide: P5
    • Protein or peptide: P6
    • Protein or peptide: P8
    • Protein or peptide: P9
    • Protein or peptide: P11
    • Protein or peptide: P12
    • Protein or peptide: P13
    • Protein or peptide: P15
    • Protein or peptide: P16
    • Protein or peptide: P17
    • Protein or peptide: P18
    • Protein or peptide: P19
    • Protein or peptide: P20
    • Protein or peptide: P21
    • Protein or peptide: MCPv5
    • Protein or peptide: P22
    • Protein or peptide: P23

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Supramolecule #1: Paramecium bursaria Chlorella virus 1

SupramoleculeName: Paramecium bursaria Chlorella virus 1 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 10506 / Sci species name: Paramecium bursaria Chlorella virus 1 / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No

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Macromolecule #1: Major capsid protein (MCP)

MacromoleculeName: Major capsid protein (MCP) / type: protein_or_peptide / ID: 1 / Number of copies: 72 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 48.199625 KDa
SequenceString: MAGGLSQLVA YGAQDVYLTG NPQITFFKTV YRRYTNFAIE SIQQTINGSV GFGNKVSTQI SRNGDLITDI VVEFVLTKGG NGGTTYYPA EELLQDVELE IGGQRIDKHY NDWFRTYDAL FRMNDDRYNY RRMTDWVNNE LVGAQKRFYV PLIFFFNQTP G LALPLIAL ...String:
MAGGLSQLVA YGAQDVYLTG NPQITFFKTV YRRYTNFAIE SIQQTINGSV GFGNKVSTQI SRNGDLITDI VVEFVLTKGG NGGTTYYPA EELLQDVELE IGGQRIDKHY NDWFRTYDAL FRMNDDRYNY RRMTDWVNNE LVGAQKRFYV PLIFFFNQTP G LALPLIAL QYHEVKLYFT LASQVQGVNY NGSSAIAGAA QPTMSVWVDY IFLDTQERTR FAQLPHEYLI EQLQFTGSET AT PSATTQA SQNIRLNFNH PTKYLAWNFN NPTNYGQYTA LANIPGACSG AGTAAATVTT PDYGNTGTYN EQLAVLDSAK IQL NGQDRF ATRKGSYFNK VQPYQSIGGV TPAGVYLYSF ALKPAGRQPS GTCNFSRIDN ATLSLTYKTC SIDATSPAAV LGNT ETVTA NTATLLTALN IYAKNYNVLR IMSGMGGLAY AN

UniProtKB: Major capsid protein

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Macromolecule #2: MCPv1

MacromoleculeName: MCPv1 / type: protein_or_peptide / ID: 2 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 58.136645 KDa
SequenceString: MPGAISQLVS YGAQDVYLTG NPQITFFKAV YRRYTNFAME SIQQTFDGTT DFGKFPTVTI SRNGDLAGPI WIEVNLPSLL GYNITPTPA EGNTSNIAAI STVFKDDYNN YWWTYNPGTT PQYSNLIAAF SNVDYKYYAN AVTSTYPPTA LSNVVYSWPY M ITGNTGTR ...String:
MPGAISQLVS YGAQDVYLTG NPQITFFKAV YRRYTNFAME SIQQTFDGTT DFGKFPTVTI SRNGDLAGPI WIEVNLPSLL GYNITPTPA EGNTSNIAAI STVFKDDYNN YWWTYNPGTT PQYSNLIAAF SNVDYKYYAN AVTSTYPPTA LSNVVYSWPY M ITGNTGTR STVAIPTANL RYVNGIGLAL FNSIELELGG QRIDKHYSEW WDIWTELTET AEKIQGYNTM VGRYDPAVYN AG WNISQAQ GGTYYVPLKF CYNRNPGLYM PLVALSYHQM KLNFNINNYL NCVKCNYPVT ALTSKNGANP LSITNMKLYT DFV FLDAPE RIRMSEIQHE YLVTQLQWQG SEPVTAPGDP NGSTNRKITL NFNHPVRELV FVYQAASNYD VDAVTGNNIF DYEI PANPT ATPPYAGGGE VFTEVKLIIN GSDRFSGRPG AYFRLVQPYE HHVRVPSKSV YVYSFALEDA DSRQPNGSAN FTRYD SVQL QLTLNENLAS GRVQIYAPNF NILRIAAGMG GLAFAN

UniProtKB: Capsid protein

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Macromolecule #3: MCPv2

MacromoleculeName: MCPv2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 55.733863 KDa
SequenceString: MDPTTRFRGS DVPVHNEDHM VFAKPSEIGL QGRQGFYTNT NVVTNTYNHV YTPPGGHTGG KFTQDIYTTD LLIAAKDEKA DKIPLDKRM DGTFSEGTFV QLLSRGPQDV YLTYNPQISL FKRKYKRYTN FAIENFEERF STVVKFGTRN ICQLSKNGDL I GNMCFRVT ...String:
MDPTTRFRGS DVPVHNEDHM VFAKPSEIGL QGRQGFYTNT NVVTNTYNHV YTPPGGHTGG KFTQDIYTTD LLIAAKDEKA DKIPLDKRM DGTFSEGTFV QLLSRGPQDV YLTYNPQISL FKRKYKRYTN FAIENFEERF STVVKFGTRN ICQLSKNGDL I GNMCFRVT LPNLGIPGGT WAPTMGYNIF STIRMRIGDT IVQSHDRVWY DIDDKMFCDS TKIQGLDELI KRNVTLTTDQ EW ELIIPLK FFCCKRNTGK QQFLPILNMD TNINVYLEFN LLPLNSLVTL PTGSILPDIQ SIDASVWVDY IFLDDTEKFR FAQ DPCVYL IEQTASMDSL SYATTTSGQT YNKTDVYVDM KELNKPVKYV EIIAYEQGST SFDYVDIIES ATFYLNSDEQ FKTR AGEYF SLVQPYQHFK RSDPTSNIST FSFALDAGSF QPNGYLNFAP YVRTRFAFKI QPQTTPHVIR LFAVCLNWVK FESGL VKLL FN

UniProtKB: Major capsid protein VP54

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Macromolecule #4: MCPv3

MacromoleculeName: MCPv3 / type: protein_or_peptide / ID: 4 / Number of copies: 11 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 45.115641 KDa
SequenceString: MSGALNQLVA VGKQDAFITG DNLDNSVWRN VTKKVSNFAM ESIENYINVN PGSTTSIVVG RFGDLVRKVT LEITMKKGST TNPFYPSEQ YVKYAHLSIG GQIVYRIDDF PTWTRVHEEL FMDPDTRATN QRVNNFRPED PVGAIRKFYL DIPLFFTDDA S KALPLIAL ...String:
MSGALNQLVA VGKQDAFITG DNLDNSVWRN VTKKVSNFAM ESIENYINVN PGSTTSIVVG RFGDLVRKVT LEITMKKGST TNPFYPSEQ YVKYAHLSIG GQIVYRIDDF PTWTRVHEEL FMDPDTRATN QRVNNFRPED PVGAIRKFYL DIPLFFTDDA S KALPLIAL QYHEVKLDVL FASPGDIPGI DASYTPTVEC YIDYVFLDTP EREWFARNTH MYVIEQLQHY NNVFSVAAVE QT KRLNLPF TMPTRYLIWL LKTGADGVYT TSGYPFENND SYAAIKSAKL QFNGTDRFSE RPGYYFTTVQ PQDAFGRSPS AGI YVYSFG LDVTGMTSKG ALNFSRMDNA TLSITTKAAT AATISDVYNQ STTLASAITD FTSIAIYAKS FNVLKIDYGM GGML FAN

UniProtKB: Capsid protein

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Macromolecule #5: MCPv4

MacromoleculeName: MCPv4 / type: protein_or_peptide / ID: 5 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 45.034797 KDa
SequenceString: MVSGGVVQLI SYGAQDVYIS GGPKTIFKSY VKKVNNFAID SVKIPINGMG NGNPSVLIPR NGDLIKSIRL EITLKKGTGQ SFYPAEQLI SYLALKIGGN IIQKIDDFSN FCRIRNELFM DPDERHTDYR VNNFSSSDVV GSVKYFFLDL PLFFSKDTSV A LPLIALQY ...String:
MVSGGVVQLI SYGAQDVYIS GGPKTIFKSY VKKVNNFAID SVKIPINGMG NGNPSVLIPR NGDLIKSIRL EITLKKGTGQ SFYPAEQLI SYLALKIGGN IIQKIDDFSN FCRIRNELFM DPDERHTDYR VNNFSSSDVV GSVKYFFLDL PLFFSKDTSV A LPLIALQY HDIELDFRFN DVSTIQGVDA SYTPTIEMYI DYVYLEQQER IKLVEKPLFY NIEQCNFYRK DITFPALSST FK LDIPFNN PSRFLVWYFN TDSFGIYNTS GLYFNSNDAA APLESAVMLL NGTERFKSRE GMYFNCVQPQ QALGRSPSSG IYM YAFGED VANATSKGAL DMSRVNELSL QLTTKNATAA NVYTVYNPQE TLASSTSTYK GITLFSTNWN VLQVRSGLGA VMFD S

UniProtKB: Capsid protein

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Macromolecule #6: P1v1

MacromoleculeName: P1v1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 57.555555 KDa
SequenceString: MDDIETIEKY GRDETYIFLA DSAKRDTAAY PTAAEYEVLF NAQFRNVTKF ELLEVNIPRT EYLIDSSENT FAYALNQPTN INTWQQEIE GNIRIATITP GDYNLPQLID EMNNVLQQTA NTYGDSVILQ VSPVTNPSEI SNKIRITASG PFTLLGSTGT I GNTIGFGD ...String:
MDDIETIEKY GRDETYIFLA DSAKRDTAAY PTAAEYEVLF NAQFRNVTKF ELLEVNIPRT EYLIDSSENT FAYALNQPTN INTWQQEIE GNIRIATITP GDYNLPQLID EMNNVLQQTA NTYGDSVILQ VSPVTNPSEI SNKIRITASG PFTLLGSTGT I GNTIGFGD PVNTSVASTT GYYSTVPGYS VNYPNGADYV FLSNQGNIGS EAVNEFVGPL PPGDNVSFTP IYTGQTPSQY FI APSAGVP TTVSAYFVDQ ATAPPGGFVV NYSIIKVSDS STIATGSLIS TNDDLVPSVS SPSVVSANFI QGQQYYIQFT PGS SGSSAG NCTALWYSFP NLPPVSGAYA AINGTLVFPG QYFCTDVSAG AYGNELTSPG IVNITGARYI KIRCKELEQL IYRD RVGEP TTAGIGIVNL IGYGFERSRY DFSSIPVKAF HPIGKLQKLT FRLERPNGTL YDTNGVDNTM LCALTFKVVP NNAVD KTFD GPGKYPAAPG YSGDYIQLQQ KRWGEEARAT YPTHKSTYNR CRPRTT

UniProtKB: DUF5901 domain-containing protein

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Macromolecule #7: P2

MacromoleculeName: P2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 63.888082 KDa
SequenceString: MCNTYYKRVK FNLFLYTNSI EMELLAVASI IGYGLFSSQQ GRETRPDRNR YAEALGSGQG LDEDYDVKPT DMVRKYRKKA EKRWKKAQV PKESGIITPN MRPSEVMPYF TSGKSMNTNT DYKQRKMELF TGGVLDGHSV SGTYKHKVEA ANMFGMTPQG R VTSDGTVG ...String:
MCNTYYKRVK FNLFLYTNSI EMELLAVASI IGYGLFSSQQ GRETRPDRNR YAEALGSGQG LDEDYDVKPT DMVRKYRKKA EKRWKKAQV PKESGIITPN MRPSEVMPYF TSGKSMNTNT DYKQRKMELF TGGVLDGHSV SGTYKHKVEA ANMFGMTPQG R VTSDGTVG NAPGDTELLK ARSVNSHQYN NVLPTEQLRV GPGLGVGPEV AATGGFHQFY RQLPLNINEY KLTQLPGRLV PG GTTTGGK GEIQQIASVN HNPDALVLNY DDRPPEATPN GAILASTQYG KQPRGYAGLR PYEKNYEGIA EADVSALQAR YLD QTRGRP RTGDGDTEPI INPNGERDGT GSYVTENMCS MTLESQRGLV NRYITPPGVT GVVQQGGEMR PEFVPETTIR EQYE DIYYT GPAGTTVTPT EPMNVVELQP ESRHAKRAGQ DRAYTPGAGR VNNFAPAAQG AYGLKDHPTY NALQHVVSEP IEQTF LPAA QGDDDRFGTK SNVNNPWGNP ASLQIANNQL AANKFNRDVT NTVNLDYDAG QPMKQQNFQP KAWIPNNTDD MKMLPL WKR KQLQAQKNKQ QRK

UniProtKB: DUF5899 domain-containing protein

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Macromolecule #8: P3

MacromoleculeName: P3 / type: protein_or_peptide / ID: 8 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 19.116688 KDa
SequenceString:
MAMKTQRKEN VLFQNVKPRE IPLVDNPFST YPYKHVITET QPTQAKNQAI WGLVQMGLSG EAAAMYGDVV VQKTTRACRK SEGGFKDVN TELWGTSPYL GRGDGEVYNM PASNQLLRGF ESSLRGSRVR TQIDDKSFIP YTWQMIDVPL AAAKTSFIAG L DTRQQLAY GNP

UniProtKB: Capsid protein

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Macromolecule #9: P4

MacromoleculeName: P4 / type: protein_or_peptide / ID: 9 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 20.637311 KDa
SequenceString:
MFSAFRDTAS IGFSDTHQDE KTLRFLKKQI SQFIKHLKEY YPNNELTKKL VMKYSDVQLL PYTKGATKDT YTSGLFDHTT GVIKIAPRD GLGNVRDEQS LNKSICHELA HGTRVKYPGE SSHSDEWKDA WKTFLKIAAD ELGWKIEVPC SSVSFYGLTK D DCENCVWD QDPETCPKTA KLA

UniProtKB: Uncharacterized protein

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Macromolecule #10: P5

MacromoleculeName: P5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 16.455834 KDa
SequenceString:
MDSRLSAAYA IRAARISMIP GGVDGLVINY AEGGEPAWVQ YPLKKQKPLP NNLCYTPTLE DIARKREAVI AKYTKQPLET GTTFTHVLN ASHLNEQYTR VKKSALPDKE FPIIETEKYP EPPILWETTI GAPSRLFDRS DGVKYVR

UniProtKB: Uncharacterized protein

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Macromolecule #11: P6

MacromoleculeName: P6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 24.036729 KDa
SequenceString: MILVGIAVLI LLAVFAILYY KQKEKFVVVG KFVEPIPSNP GQDFTLLPMD QTYTFADPVP DTATAFDVVL SRFTDKKAPA DLLKGATFP EAAPYTDSEV ENISKLALSR VKGPDAPVLS FISVEYAAKG VDNKKNTHYD IAFMVYDQVK NFSLKLVLVA V LDAKNKLW ...String:
MILVGIAVLI LLAVFAILYY KQKEKFVVVG KFVEPIPSNP GQDFTLLPMD QTYTFADPVP DTATAFDVVL SRFTDKKAPA DLLKGATFP EAAPYTDSEV ENISKLALSR VKGPDAPVLS FISVEYAAKG VDNKKNTHYD IAFMVYDQVK NFSLKLVLVA V LDAKNKLW IKKFSSFNSF TPKDKGPKGV ENIDETPLAE FIPDFVQFSR LYKDNANV

UniProtKB: PsbP C-terminal domain-containing protein

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Macromolecule #12: P8

MacromoleculeName: P8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 19.226947 KDa
SequenceString:
METIGNFIQN MAFPTENPYK LSGDFGNSTL EPTVTAALRI LHESPAPFNT EFFSRKNVDF IQKKLISETK RYTSFDIGPQ NEDELIMMM AGIYVQDSTF DGNIKAGLRK LNTLVITECL KQILPGVRAY ALYVRDASLP FSGGGEEAFK RPELATLKGS K VLSGFLPL DRNAS

UniProtKB: Capsid protein

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Macromolecule #13: P9

MacromoleculeName: P9 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 23.407564 KDa
SequenceString: MKTETLYQDP VWFQDLSVIF KRPSEIIPTR DQSDSERINA MVRLVLYCSF AVALIRQNYL YAILGLAIIA IISLAYALGA KKTKSNEAY GNIRPYSVKR SKKSCSKSSA NNPFSNATVG ALLDNEARPP ACSYDDNDMA STMRKNFNKG LFRNLDDVYE V ENSQRQFY ...String:
MKTETLYQDP VWFQDLSVIF KRPSEIIPTR DQSDSERINA MVRLVLYCSF AVALIRQNYL YAILGLAIIA IISLAYALGA KKTKSNEAY GNIRPYSVKR SKKSCSKSSA NNPFSNATVG ALLDNEARPP ACSYDDNDMA STMRKNFNKG LFRNLDDVYE V ENSQRQFY TMPVTTAAPD LTAFGQFLYG SKGKTCKEDP SACTPAFATR DG

UniProtKB: SAYSvFN domain-containing protein

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Macromolecule #14: P11

MacromoleculeName: P11 / type: protein_or_peptide / ID: 14 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 23.322656 KDa
SequenceString: MDMHMIVKVV AILAVLFLVY KLWESMNKPN ASPLKIQNPY EKYMNSAEGG EYDAEDDDIY YPETDAEDDD IYTGETDDMY DGEDDDIYV QEGDDIEDAE DEPYDDSADM EQDVPKVQQP MMPLLTPSSQ LLPKPSPEAA DFAQFAPKNL QAQNFLTATQ W IGVNTQGS ...String:
MDMHMIVKVV AILAVLFLVY KLWESMNKPN ASPLKIQNPY EKYMNSAEGG EYDAEDDDIY YPETDAEDDD IYTGETDDMY DGEDDDIYV QEGDDIEDAE DEPYDDSADM EQDVPKVQQP MMPLLTPSSQ LLPKPSPEAA DFAQFAPKNL QAQNFLTATQ W IGVNTQGS SLKNANYDLR ADPIIPKADV GPWMMSSVDP NIYQKPLFG

UniProtKB: Uncharacterized protein

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Macromolecule #15: P12

MacromoleculeName: P12 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 17.715475 KDa
SequenceString:
MGNGPPMERA VSSDDILTYY NTFIFFIYFN FTNENIYIIY TIYMKVQNTI VYIVLLLIVV VIIWNFTRKE GWSDYNAPND FMKIYYSNI VEDKKLAEKY PFFGTGPFTG LRCRKPNNVG CNTTWVSGQL VELTPKLKEQ IECKFGIQYV KT

UniProtKB: Uncharacterized protein

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Macromolecule #16: P13

MacromoleculeName: P13 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 11.07083 KDa
SequenceString:
MHKITPFLIA AVVAVIVLAV WLFKKDNKKE TWFSRDLNYG KANSKIWNAT VAKGLKGIAN ENAEIRKMYP YLGYGDFTGA ICKGPNNQG CTYYANYTR

UniProtKB: Uncharacterized protein

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Macromolecule #17: P15

MacromoleculeName: P15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 18.627422 KDa
SequenceString:
MWLFFFALAV IYMIYKRDVF KKIAVNLKMN GVSIPFVDKY SKQYPTYTKN ALFHVTRFNN AYQKTFEYKN ISIDTINNLF SIRDDVLYN ISEIKLRLPN DLTQEKEINY MYEKTDQRLM EYITDVKSRF HINIYPGTMS SAFEARNYRA SNDIVF

UniProtKB: Entry/fusion complex component

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Macromolecule #18: P16

MacromoleculeName: P16 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 9.844584 KDa
SequenceString:
MQGGLFGTIK LMIMLFSYFA AYQLGKMQER PQSQWPKAKA GQNKYMVGDW AAWKPIYMGV LGVAVLLTLL GPGGVGGGMG GMFGGGGGY GGYY

UniProtKB: Glycine-rich protein

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Macromolecule #19: P17

MacromoleculeName: P17 / type: protein_or_peptide / ID: 19 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 9.12237 KDa
SequenceString:
MGAFTSFVLM LLFTGIILIA TNELTYNRPR EIQYRYLPRD LDSFIRTQEM PSAIFSSMWD VDTRRGGDGG PNPPGIRQSN

UniProtKB: Uncharacterized protein

+
Macromolecule #20: P18

MacromoleculeName: P18 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 16.497771 KDa
SequenceString:
MTTITYDTDL LPPPELKVPS LDQALAPESV KNDDPFLDLS YFPVPKGFDN VGSLELNNLS TAEDVATLQN QLNKLAEEKH KRSTWKGLT FRIAVQDMWE ALTGIPTDIY QNSGRVSLKE LLTRDDRLRG LGLIFFLVAV VSIFFLAAG

UniProtKB: Transmembrane protein

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Macromolecule #21: P19

MacromoleculeName: P19 / type: protein_or_peptide / ID: 21 / Number of copies: 25 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 43.094387 KDa
SequenceString: MFLYYKMNEV LNVNNGSSIN VIKILKTPGP DIIRPGKTYK KKDVFTPKFF KNGNVMYTCN TFSLNVPVNN SIATIDFAEH VNGAVFKIE YNRVNFIAPS LYPISGLGTA VVFDLQKGEK ASQRKITEFG NKDIRIADEI SDIAADDHSV LITTKLMSES T PGELSRDV ...String:
MFLYYKMNEV LNVNNGSSIN VIKILKTPGP DIIRPGKTYK KKDVFTPKFF KNGNVMYTCN TFSLNVPVNN SIATIDFAEH VNGAVFKIE YNRVNFIAPS LYPISGLGTA VVFDLQKGEK ASQRKITEFG NKDIRIADEI SDIAADDHSV LITTKLMSES T PGELSRDV VLNGEIATGR INMNTGFVSD IIHTKKISII DDGIVDYYVK INVPAKYSHG IVEVVSGTFL NDIMIHLVRN KN KWALMDS KMYIVNNTNG FVIAKNPDTA MGVSLLEWPR GAVVFPPYYN FKKFKNVNKW SISQQIASCV NTSVKIPGGE YSW KIRLFF GPIWQVQESI NSVKTVPHGN DYMYLYKEEI VKYKYQRNTK RNFHEYDYDY KF

UniProtKB: A2M domain-containing protein

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Macromolecule #22: P20

MacromoleculeName: P20 / type: protein_or_peptide / ID: 22 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 137.671312 KDa
SequenceString: MSGYSNPLND PIMIRTMIVY GNLSATYFTG NGAALTQQPI ASNVPPTLRA DAFGNVTSPA NVISGNITTI DVISAGNINT RYVFGDGST LSGISTGNSV VGVQKIDIIG NAVSSTISTG SLRTNVAFTN ITANISNISY LSGNTVSNII NASNLIANIV N ANVMSGDI ...String:
MSGYSNPLND PIMIRTMIVY GNLSATYFTG NGAALTQQPI ASNVPPTLRA DAFGNVTSPA NVISGNITTI DVISAGNINT RYVFGDGST LSGISTGNSV VGVQKIDIIG NAVSSTISTG SLRTNVAFTN ITANISNISY LSGNTVSNII NASNLIANIV N ANVMSGDI TLNRIVATDT RTINLFGNVS TNSFTAYGNV TANILTATTL TANSLQIESV SGNSVQKMIV GNLIGNVLTA RT ATFDTAN IPTITSNVGI FTVANIRTIT GDVRANVLNV SNIVTRSIGT DVVSNNIIAT IGNVQSMIIG NLSANTLSVS NGT FQNFTT TVGLNVTTLS ASVSNALVLS FTTGNSSSTT TTNLDAYGNT QFLRISTETS GINILNANSV SVGNLTIVGN VTST SNNIS GQVTSNSSQV QNINTNVIYS GNVFTSRFIA ANANIRSLTA SIGQVNSLTI TSGNLTSLSF VNGNGNGVIA TNANI SNLN AVSMSGFAFD ANVIIGDITA NILSSGNLNA NVFEANMLTS GNVVSTNGDT GTINANSIVL NVISGGDTRI DRLIAN SVT TGDFYTTSTI TAGVIDCAQG KISGNSSSNA SLISNVISAN TASFVNTSVG YGNLVNAVVI GNIIANVVTS GDANAVF LN SNLTTSGSIF GNISNSASIV AEYSNIRTMN VGNINSTTFN AEYLYVGNIT VTGVSNTGYL ICRNATFSLN SNIQTLIT E NVTTSNAGSN IFTNVITTGN AFSQNINTIN ANVLGITSVF SNVITNISNI RTLILLSNVS LGNLTATSAN VTSNVSVGN LIGNIVASNA FFNTATIRNL AIFDVTGNIL VAQSNASISN LTTERILLSD ANIANLSTSN VSSNIAVVTG TCRIDAINSS NITFTNTAT VSAAVVNVLG NLTVSNVINV QNLTANVGNI WQAVSNIQSM TTANIFTNTM TIINSFTYSN ISSQRLIATN T LTANGVNL FSNIANLTVS GNANVGRWIA ASAPATGALC FSSTESANLT TANLFAGNLN TWRAFVGNVI GNVMTTVAGD FG GIRVSVA NIDVSGVANV ENFTVNGNLF ATNVITRDLS ANLINFSGSL SNTSSISISG NSLFTSSANA ANVGVSINMI TQN LFGNRL SSENIQGNVL QASNIAVSQT VNVVGNISIG NLSVSRELFA NSITISSGGT IVGNNTTRMI TTPTSNSWSV GSLV GNANV SVQFTANTMV VQNIQPNTLV VNINQSTPTS NSGVRAIYIG YIVPVNFNAT SSTTVSFSPA LPTNCIYMPQ LQYYS GGTV LQNNASFSTQ IGLGVETITN TSCTIRMSTL DTLPIPSTSF FGLMVTAVQT V

UniProtKB: Peptidase S74 domain-containing protein

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Macromolecule #23: P21

MacromoleculeName: P21 / type: protein_or_peptide / ID: 23 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 141.414188 KDa
SequenceString: MSWFDPNWNP FFNRSIIVSG NLVVAGGVIE GNGGGLTNSA FSNPDTIRTD LTGNVTSNGT VRSSLIQTNS IVASGDAYAN VWNGNAAFI TGISANTTLV GNVRISISGN LTGNFAVSTT TVSNTMTANV LIGTTLTVNN LSATRANVQV LTGNVTGNVR L IANVVNVA ...String:
MSWFDPNWNP FFNRSIIVSG NLVVAGGVIE GNGGGLTNSA FSNPDTIRTD LTGNVTSNGT VRSSLIQTNS IVASGDAYAN VWNGNAAFI TGISANTTLV GNVRISISGN LTGNFAVSTT TVSNTMTANV LIGTTLTVNN LSATRANVQV LTGNVTGNVR L IANVVNVA AYTVGNLTTS SNVSTTNLNV TSNINGNVRS STANVQGRLS ATTPTTGSII SSSFIANSFT SSGNVQIGDF FG NVRASVG TFDMIASNVF SNVANIIEIR SDIISNIANV GNGTFVFVNA SNITTTNITG NITSNVASFS NVDSIFGNVG NLR ANTLTV TYANITGTLA SGNVAVGNLI SADNTSEFAN ITSFTTNGLV VNGNIIAAIG NGNVFLANVI TANSLTLQSS SSTA ASNVT TTRWIQSTTS TSNVMTIGNI TSGNVISSNA AYFANVISNT ITVMSAMTVS GNVSFGNVSF GNVSYGNITA NVTTS TGNV SVDNLTSNVV NVVFCNASSV TANTLTSIAL TGNTYVYSAN TGVLTANVGN VFGTLTVGVA NVTSMVSNAL FANTAV VSN LSVSNLTVTI SSDISNISIT GNTSGNVFAA NIATIGTLNT ANVVANLVAS NVMNAWVTSN IRTLITGNAN VSTTTSN VI TTGGFTITGN ITSGLLTSNV IAGNITMYNT SNTTLFTSNT SNIANFFAGN MTAVNTIVSN LEIQGNSVVI TQTTPFKV T SVLLANVLFA NTIISNTFTT TANVVGNIST DIANIGIANV NFLNTTDFAV NTANIVNYTP TSNINVTGNL TLGNANIVN FYATSANIVG NITANNAVIT FLTTPFYKGS GTVSAGFTSI ISANALSNVI VFGNLSANIV VSNTLLSNSI NVATVFSNVV NIGQVTSTG TTLVNSINFN ISNVDVSGNV LVTRDVYTTN ISVTTANIAN VTFFSNVAIG NLISTRNLTA ANLISSSDLF N SGPYTSSQ NVTIDTLNFT AGNLGTVAAR TTITTPTLFA TDVNFTQDAL VAGELITQNF YGNITSANIG SRITIGNANV TQ TNITGNV VIPRTSNAAG FNSRLLISNN TTVTSNIVAN SLISTGNIIT NLLQTTGQVS FASLAVEYID VANLAVRNVV TIG GNLTVS NVANLFSITA NTVNMSTVTT NTLSANTITG TSNVLVAGNI IGNCFGNVLV NRGVVTGNVF ADTITVPLNA VGVL TGNAL IVPNTALHSV AITGSSAFTS NIRTLNTPNA FIWNTSVPSS GLLDARRLRF SQYITSNRIV EMRYFYITNA LPQKY DSGG LNARYDLSFG TTLPTSQGQT WHHFLNTLYI GAQYNTNFSI NCLIINATTT GLEVIIYNPF GIAASLSSST PELYFY ITS IATSTQ

UniProtKB: Chlorovirus glycoprotein repeat domain-containing protein

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Macromolecule #24: MCPv5

MacromoleculeName: MCPv5 / type: protein_or_peptide / ID: 24 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 45.585547 KDa
SequenceString: MGGIMQLVST GYQDVFLTGD PTRSLWKRIM ARKTNFAIES IETVFDVLYG STSFINIKKA GDLLKSCVLE ITMKRTSTES FYPAEQFIK SLTLLIGDQE VEKIQDFPTW SRVHDELFND TEMRSANYRM LNFRPDDPPG AIRTFYLDLP LFFTRYLSNS L PLVALQYH ...String:
MGGIMQLVST GYQDVFLTGD PTRSLWKRIM ARKTNFAIES IETVFDVLYG STSFINIKKA GDLLKSCVLE ITMKRTSTES FYPAEQFIK SLTLLIGDQE VEKIQDFPTW SRVHDELFND TEMRSANYRM LNFRPDDPPG AIRTFYLDLP LFFTRYLSNS L PLVALQYH EVKLKIDFEP PYNIPGIDST YMPQVRFYGD YVFLDKPERV YFAQTEHEYI IEQLQTFVTI PNMSNSVNTT MV DLPFTLP SRYIIWVYKT NLHGQYTTSN STFVTNEGYA PLQSAVIKCN GTDRFSERPG GYFNMVQTTQ ALGQAPSCGI YMY SFGVNA DKQDPEGTLN FSKLDMVTLS YTNKAATAAD ISQVFDLSTT LENGITKFKN ITIFSKNFNV LRIVEGQGGI LFSN

UniProtKB: Capsid protein

+
Macromolecule #25: P22

MacromoleculeName: P22 / type: protein_or_peptide / ID: 25 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 137.927828 KDa
SequenceString: MGSYFVPPAN YFFKDIFASN VGNIANVIFD NGNVIAAGGL GYLIGNGAFI TGVTSTAIAN IPAVVTADIR GNLIGNYANV NNIIASSGN ISNVRFVSGG NVTASYYFGD GSQLTGITAT ANIPSIVTAD IRGNIIGNYA NVSNVSATFG NIANVLFNNG N VTAAGGNG ...String:
MGSYFVPPAN YFFKDIFASN VGNIANVIFD NGNVIAAGGL GYLIGNGAFI TGVTSTAIAN IPAVVTADIR GNLIGNYANV NNIIASSGN ISNVRFVSGG NVTASYYFGD GSQLTGITAT ANIPSIVTAD IRGNIIGNYA NVSNVSATFG NIANVLFNNG N VTAAGGNG FFIGNGSLLT GITATANIPS IVTADIRGNI IGNYANVSNV SATFGNIANV LFNNGNVTAA GGNGYFFGNG AL LTGITAT ANIPSIVTAD IRGNIIGNYA NVSNVSATFG NIANVLFNNG NVTAAGGNGY FFGNGALLTG ITATANIPSI VTA DIRGNI IGNYANVSNV SATFGNIANV LFNNGNVTAA GGNGFFIGNG SLLSGITATA NIPSIVTADI RGNIIGNYAN VSNV TATFG NIANVLFNNG NVTAAGGNGY FFGNGSQLTG VTATLPSIVT ADIRGNIIGN YANVSNVIAT FGNIANVLFN NGNVT AADG NGYFFGNGSQ LTGVTATLPS IVTADIRGNI IGNYANVSNV IATFGNIANV LFNNGNVTAA GGNGYFFGNG ALLTGI TAT ANIPSIVTAD IRGNIIGNYA NVSNVIATFG NIANVLFNNG NVTAADGNGY FFGNGSQLTG VTATLPSIVT ADIRGNI IG NYANVSNVIA TFGNIANVLF NNGNVTAAGG NGYFFGNGAL LTGITATANI PSIVTADIRG NIIGNYANVS NVTATFGN I ANVLFNNGNV TAAGGNGYFF GNGSQLTGVT ATLPSIVTAD IRGNIIGNYA NVSNVIATFG NIANVLFNNG NVTAAGGNG YFFGNGALLT GITATANIPS IVTADIRGNI IGNYANVSNV TATFGNIGNV LFNNGNVTAA GGNGYFFGNG TFLNFSTITA DIRGNIIGN YANVGNVIAG NVSTTLGNIG NVLFNNGNVT AAGGNGYFFG NGTSLTFSTI RADIRGNIIG NYANVANVIA G NVNSTFGN IAGVTFDAGN VSSPVDILVS GNVSVGSDGL FRGPTNQSNN ALILRGIGGT NTVNLFSIGA PSGQMRFSVP AS GVAFYNF LYGTNQVASL GPQGFYSLSY DSSVVVGQVV SNSTTYSGTI FKTEANRNSS TAFNHISCTG SNGNVFRVRG DGT TYGVGA FQTSGADYAE MMEWEDKNPT FEDRRGYPVV LSGNGKIRIA SLSDDYNNII GVVSSNPSML ADMAWDQWEG KYLK DKFGM RLSNTIYYLA NISNENEFTR VGPMTTPPQG YFIKTSPEYI LNPKYNPNVA YVERNDRPEW DPVGFVGKLR VRNGC LVHP SWKPLKVIDD YADVGNAAAQ VTEYLIGVTP NYTLSKKVED LEQTINVLKT QVQMLLGNI

UniProtKB: Peptidase_G2 domain-containing protein

+
Macromolecule #26: P23

MacromoleculeName: P23 / type: protein_or_peptide / ID: 26 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Paramecium bursaria Chlorella virus 1
Molecular weightTheoretical: 140.121688 KDa
SequenceString: MASGWSDFRA DPVIVNQDVV VFGNVFVDYL IGNASEMTGA ADFVPGNVVN VNIIGNTVST FVKSDVLMGD TITIDGNITA TRLYGDASN MSGLPTLPPN ANIDMVGNIV ANTNTNKFVS VDTFTGDVTT NVATISNANV GTVTTNTTFF GNVITNVFNI G NLILTSSN ...String:
MASGWSDFRA DPVIVNQDVV VFGNVFVDYL IGNASEMTGA ADFVPGNVVN VNIIGNTVST FVKSDVLMGD TITIDGNITA TRLYGDASN MSGLPTLPPN ANIDMVGNIV ANTNTNKFVS VDTFTGDVTT NVATISNANV GTVTTNTTFF GNVITNVFNI G NLILTSSN VDTLNVTSLV ASNVVSSQVD VQATIANSDL NVTRFDATSI SAEIFVSSET LAVSNITNLI ANIANIQGTT TV TGNLVGN IANVNTLTGN VTGNLFFTNL IIGNITANIF NTTTANVSNL ITSNVSASNV SASNVTSTLF TIGNLNTNVA TIS NLFVTG TTIAGNTTFS SFSVSSLVAN ISGNNASIPT FTPNVLSTSN ITIDGNAVVT GNAGFRTLNV TSLTANVLTT RGDV SNLYT DVINVNTNVF ISSANILANN VITVQMNNIS NVNITGNAEA FDVSANSVLM STLSVGGILS LGNVNTNDIS FGNIV TGNF FANFANVGNV QGNANVITMY STTLTSNSAQ IGNISGFSIV SNLGNAVSMF VGNLNTGTLV ANNSTVGNIT YISGNI GNA NAQTVRVSGL LSASGVALTF NSITSNVAGN SLTVHGNLRT NSVTTEILTG NVGNLSILNT GNLFANSISV GQSFVIS NF ANAFIISSQT TNASVITANI SGNIRVTNTL EANVVNSDSA NIIIKNVSTS GNVSNTFTTG NLTVTNAVFR SGITGNNI E VVGNVNCDGN TTVTTLSYGN LITSVYNTRN IFGNITANTV NITNSNIGNV VTSNINANSL IITNLIPTVD ITANVLRAS QVNTGWFYGN ATCNVLSMSN SVSINTLLSA EVFTSETINA NANVSVATIN SLGNTVAISR PSFLGNANIR LVISNVITIG NLTANTLVG DSFTATGIVN AINSNSQSDS IATNVTGNII IRGNLILASS ASAQSNASVF NPTNLISLNA NSLSSLTATN V SANNVVIS NTWQVSNLIV SNISAPNVTA NSFQAISMTG RYYRPGISIE RAGNLIVSNS LTSTANILQV LTSNTGQVNI TN TMFGNIS MSNIIVTGVV VTGNLTMGNV IANAVTTSVS TITGNATFGN LTVTDAVIIR GNITANNIST GSYIYGNGAF ITG NLTSSG NANISIATRM FGTNSAINVA GTFTGVGNTN TTNATIANLT TNTLLSIGNL STTGNFTVNS LSVGNLFANG STIQ LSNLA NIQVSNLIYS GNVVVSNLSA NTLSYVQNIG TSTQLQSGTM NVNNILSAGR FRLSISSTAP VFAGWTMGRV SLSGQ SLRT NSGNPRTITF TTPLPAGYSY GIQLSVYSTV FGQTMALFNI TVVSLTNTSV QFYIWNNPGG SGDSNTYIGY FAYAY

UniProtKB: Chlorovirus glycoprotein repeat domain-containing protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average exposure time: 8.0 sec. / Average electron dose: 24.4 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

5384

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: PROJECTION MATCHING / Software - Name: jspr
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 56500

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