EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure and molecular mechanism of abscisic acid transporter ABCG25.
ジャーナル・号・ページ	Nat Plants, Vol. 9, Issue 10, Page 1709-1719, Year 2023
掲載日	2023年9月4日
著者	Xiaowei Huang / Xue Zhang / Ning An / Minhua Zhang / Miaolian Ma / Yang Yang / Lianyan Jing / Yongfei Wang / Zhenguo Chen / Peng Zhang /
PubMed 要旨	Abscisic acid (ABA) is one of the plant hormones that regulate various physiological processes, including stomatal closure, seed germination and development. ABA is synthesized mainly in vascular ...Abscisic acid (ABA) is one of the plant hormones that regulate various physiological processes, including stomatal closure, seed germination and development. ABA is synthesized mainly in vascular tissues and transported to distal sites to exert its physiological functions. Many ABA transporters have been identified, however, the molecular mechanism of ABA transport remains elusive. Here we report the cryogenic electron microscopy structure of the Arabidopsis thaliana adenosine triphosphate-binding cassette G subfamily ABA exporter ABCG25 (AtABCG25) in inward-facing apo conformation, ABA-bound pre-translocation conformation and outward-facing occluded conformation. Structural and biochemical analyses reveal that the ABA bound with ABCG25 adopts a similar configuration as that in ABA receptors and that the ABA-specific binding is dictated by residues from transmembrane helices TM1, TM2 and TM5a of each protomer at the transmembrane domain interface. Comparison of different conformational structures reveals conformational changes, especially those of transmembrane helices and residues constituting the substrate translocation pathway during the cross-membrane transport process. Based on the structural data, a 'gate-flipper' translocation model of ABCG25-mediated ABA cross-membrane transport is proposed. Our structural data on AtABCG25 provide new clues to the physiological study of ABA and shed light on its potential applications in plants and agriculture.
リンク	Nat Plants / PubMed:37666961
手法	EM (単粒子)
解像度	2.81 - 3.08 Å
構造データ	35145 8i38 EMDB-35145, PDB-8i38: Cryo-EM structure of abscisic acid transporter AtABCG25 in inward conformation 手法: EM (単粒子) / 解像度: 2.9 Å 35146 8i39 EMDB-35146, PDB-8i39: Cryo-EM structure of abscisic acid transporter AtABCG25 with ABA 手法: EM (単粒子) / 解像度: 2.85 Å 35147 8i3a EMDB-35147, PDB-8i3a: Cryo-EM structure of abscisic acid transporter AtABCG25 in outward conformation 手法: EM (単粒子) / 解像度: 3.04 Å 35148 8i3b EMDB-35148, PDB-8i3b: Cryo-EM structure of abscisic acid transporter AtABCG25 in nanodisc 手法: EM (単粒子) / 解像度: 3.08 Å 35149 8i3c EMDB-35149, PDB-8i3c: Cryo-EM structure of abscisic acid transporter AtABCG25 with CHS 手法: EM (単粒子) / 解像度: 2.85 Å 35150 8i3d EMDB-35150, PDB-8i3d: Cryo-EM structure of abscisic acid transporter AtABCG25 手法: EM (単粒子) / 解像度: 2.81 Å
化合物	ChemComp-A8S: (2Z,4E)-5-[(1S)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid / アブシジン酸 / ホルモンYM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト
由来	arabidopsis thaliana (シロイヌナズナ)
キーワード	MEMBRANE PROTEIN / abscisic acid / transport / ABC transporter / plant hormone