EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The nucleoplasmic phase of pre-40S formation prior to nuclear export.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 50, Issue 20, Page 11924-11937, Year 2022
掲載日	2022年11月11日
著者	Jingdong Cheng / Benjamin Lau / Matthias Thoms / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann /
PubMed 要旨	Biogenesis of the small ribosomal subunit in eukaryotes starts in the nucleolus with the formation of a 90S precursor and ends in the cytoplasm. Here, we elucidate the enigmatic structural ...Biogenesis of the small ribosomal subunit in eukaryotes starts in the nucleolus with the formation of a 90S precursor and ends in the cytoplasm. Here, we elucidate the enigmatic structural transitions of assembly intermediates from human and yeast cells during the nucleoplasmic maturation phase. After dissociation of all 90S factors, the 40S body adopts a close-to-mature conformation, whereas the 3' major domain, later forming the 40S head, remains entirely immature. A first coordination is facilitated by the assembly factors TSR1 and BUD23-TRMT112, followed by re-positioning of RRP12 that is already recruited early to the 90S for further head rearrangements. Eventually, the uS2 cluster, CK1 (Hrr25 in yeast) and the export factor SLX9 associate with the pre-40S to provide export competence. These exemplary findings reveal the evolutionary conserved mechanism of how yeast and humans assemble the 40S ribosomal subunit, but reveal also a few minor differences.
リンク	Nucleic Acids Res / PubMed:36321656 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 7.4 Å
構造データ	32792 7wtn EMDB-32792, PDB-7wtn: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-1 (with Rps2) 手法: EM (単粒子) / 解像度: 3.4 Å 32793 7wto EMDB-32793, PDB-7wto: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-1 (without Rps2) 手法: EM (単粒子) / 解像度: 3.5 Å 32794 7wtp EMDB-32794, PDB-7wtp: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (with Rps2) 手法: EM (単粒子) / 解像度: 3.8 Å 32795 7wtq EMDB-32795, PDB-7wtq: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (without Rps2) 手法: EM (単粒子) / 解像度: 3.7 Å 32796 7wtr EMDB-32796, PDB-7wtr: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-3 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-32797: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Rrp12-A 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-32798: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Rio2-C 手法: EM (単粒子) / 解像度: 4.8 Å 32799 7wts EMDB-32799, PDB-7wts: Cryo-EM structure of a human pre-40S ribosomal subunit - State UTP14 手法: EM (単粒子) / 解像度: 3.2 Å 32800 7wtt EMDB-32800, PDB-7wtt: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (with CK1) 手法: EM (単粒子) / 解像度: 3.1 Å 32801 7wtu EMDB-32801, PDB-7wtu: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (without CK1) 手法: EM (単粒子) / 解像度: 3.0 Å 32802 7wtv EMDB-32802, PDB-7wtv: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A2 手法: EM (単粒子) / 解像度: 3.5 Å 32803 7wtw EMDB-32803, PDB-7wtw: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A3 手法: EM (単粒子) / 解像度: 3.2 Å 32804 7wtx EMDB-32804, PDB-7wtx: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B1 手法: EM (単粒子) / 解像度: 3.1 Å 32806 7wtz EMDB-32806, PDB-7wtz: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B2 手法: EM (単粒子) / 解像度: 3.0 Å 32807 7wu0 EMDB-32807, PDB-7wu0: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B3 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-32808: Cryo-EM structure of a human pre-40S ribosomal subunit - State RIOK2-D2 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-32809: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (combined together, COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-32810: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (with CK1, COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-32811: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (without CK1, COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-32812: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A2 (COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-32813: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A3 (COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-32814: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B1 (COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-32815: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B2 (COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-32816: Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B3 (COMPOSITE MAP) 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン
由来	saccharomyces cerevisiae (パン酵母) homo sapiens (ヒト)
キーワード	RIBOSOME / ribosome biogenesis / 40S ribosome