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-Structure paper
タイトル | Cryo-EM structures of the human surfactant lipid transporter ABCA3. |
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ジャーナル・号・ページ | Sci Adv, Vol. 8, Issue 14, Page eabn3727, Year 2022 |
掲載日 | 2022年4月8日 |
著者 | Tian Xie / Zike Zhang / Jian Yue / Qi Fang / Xin Gong / |
PubMed 要旨 | The adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter ABCA3 plays a critical role in pulmonary surfactant biogenesis. Mutations in human ABCA3 have been recognized as the most ...The adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter ABCA3 plays a critical role in pulmonary surfactant biogenesis. Mutations in human ABCA3 have been recognized as the most frequent causes of inherited surfactant dysfunction disorders. Despite two decades of research, in vitro biochemical and structural studies of ABCA3 are still lacking. Here, we report the cryo-EM structures of human ABCA3 in two distinct conformations, both at resolution of 3.3 Å. In the absence of ATP, ABCA3 adopts a "lateral-opening" conformation with the lateral surfaces of transmembrane domains (TMDs) exposed to the membrane and features two positively charged cavities within the TMDs as potential substrate binding sites. ATP binding induces pronounced conformational changes, resulting in the collapse of the potential substrate binding cavities. Our results help to rationalize the disease-causing mutations in human ABCA3 and suggest a conserved "lateral access and extrusion" mechanism for both lipid export and import mediated by ABCA transporters. |
リンク | Sci Adv / PubMed:35394827 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.3 - 6.3 Å |
構造データ | EMDB-32233, PDB-7w01: EMDB-32234, PDB-7w02: EMDB-32235: Cryo-EM map of nucleotide-free ABCA3 in LMNG/CHS |
化合物 | ChemComp-NAG: ChemComp-PX4: ChemComp-POV: ChemComp-ATP: ChemComp-MG: |
由来 |
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キーワード | TRANSLOCASE / ABC transporter / MEMBRANE PROTEIN |