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-Structure paper
タイトル | C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 6294, Year 2021 |
掲載日 | 2021年11月2日 |
著者 | Takahiro Mori / Takuto Kumano / Haibing He / Satomi Watanabe / Miki Senda / Toshio Moriya / Naruhiko Adachi / Sanae Hori / Yuzu Terashita / Masato Kawasaki / Yoshiteru Hashimoto / Takayoshi Awakawa / Toshiya Senda / Ikuro Abe / Michihiko Kobayashi / |
PubMed 要旨 | C-Glycosides, in which a sugar moiety is linked via a carbon-carbon (C-C) bond to a non-sugar moiety (aglycone), are found in our food and medicine. The C-C bond is cleaved by intestinal microbes and ...C-Glycosides, in which a sugar moiety is linked via a carbon-carbon (C-C) bond to a non-sugar moiety (aglycone), are found in our food and medicine. The C-C bond is cleaved by intestinal microbes and the resulting aglycones exert various bioactivities. Although the enzymes responsible for the reactions have been identified, their catalytic mechanisms and the generality of the reactions in nature remain to be explored. Here, we present the identification and structural basis for the activation of xenobiotic C-glycosides by heterocomplex C-deglycosylation enzymes from intestinal and soil bacteria. They are found to be metal-dependent enzymes exhibiting broad substrate specificity toward C-glycosides. X-ray crystallographic and cryo-electron microscopic analyses, as well as structure-based mutagenesis, reveal the structural details of these enzymes and the detailed catalytic mechanisms of their remarkable C-C bond cleavage reactions. Furthermore, bioinformatic and biochemical analyses suggest that the C-deglycosylation enzymes are widely distributed in the gut, soil, and marine bacteria. |
リンク | Nat Commun / PubMed:34728636 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.4 - 2.85 Å |
構造データ | EMDB-30808, PDB-7drd: EMDB-30809, PDB-7dre: PDB-7bvr: PDB-7bvs: PDB-7exb: PDB-7exz: |
化合物 | ChemComp-GOL: ChemComp-MN: ChemComp-EPE: ChemComp-HOH: ChemComp-SO4: |
由来 |
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キーワード | LYASE / C-deglycosylation / sugar phosphate isomerase/epimerase / BIOSYNTHETIC PROTEIN / C-deglycosylase / sugar-isomerase-like |