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-Structure paper
タイトル | Nodavirus RNA replication crown architecture reveals proto-crown precursor and viral protein A conformational switching. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 120, Issue 5, Page e2217412120, Year 2023 |
掲載日 | 2023年1月31日 |
著者 | Hong Zhan / Nuruddin Unchwaniwala / Andrea Rebolledo-Viveros / Janice Pennington / Mark Horswill / Roma Broadberry / Jonathan Myers / Johan A den Boon / Timothy Grant / Paul Ahlquist / |
PubMed 要旨 | Positive-strand RNA viruses replicate their genomes in virus-induced membrane vesicles, and the resulting RNA replication complexes are a major target for virus control. Nodavirus studies first ...Positive-strand RNA viruses replicate their genomes in virus-induced membrane vesicles, and the resulting RNA replication complexes are a major target for virus control. Nodavirus studies first revealed viral RNA replication proteins forming a 12-fold symmetric "crown" at the vesicle opening to the cytosol, an arrangement recently confirmed to extend to distantly related alphaviruses. Using cryoelectron microscopy (cryo-EM), we show that mature nodavirus crowns comprise two stacked 12-mer rings of multidomain viral RNA replication protein A. Each ring contains an ~19 nm circle of C-proximal polymerase domains, differentiated by strikingly diverged positions of N-proximal RNA capping/membrane binding domains. The lower ring is a "proto-crown" precursor that assembles prior to RNA template recruitment, RNA synthesis, and replication vesicle formation. In this proto-crown, the N-proximal segments interact to form a toroidal central floor, whose 3.1 Å resolution structure reveals many mechanistic details of the RNA capping/membrane binding domains. In the upper ring, cryo-EM fitting indicates that the N-proximal domains extend radially outside the polymerases, forming separated, membrane-binding "legs." The polymerase and N-proximal domains are connected by a long linker accommodating the conformational switch between the two rings and possibly also polymerase movements associated with RNA synthesis and nonsymmetric electron density in the lower center of mature crowns. The results reveal remarkable viral protein multifunctionality, conformational flexibility, and evolutionary plasticity and insights into (+)RNA virus replication and control. |
リンク | Proc Natl Acad Sci U S A / PubMed:36693094 / PubMed Central |
手法 | EM (サブトモグラム平均) / EM (単粒子) |
解像度 | 3.1 - 17.3 Å |
構造データ | EMDB-29216: Nodavirus RNA replication crown from baculovirus-expressed viral protein A plus RNA1 template EMDB-29217: Nodavirus RNA replication proto-crown from baculovirus-expressed viral protein A minus RNA1 template EMDB-29218: Nodavirus RNA replication crown from flock house virus-infected cells EMDB-29289, PDB-8fm9: EMDB-29290, PDB-8fma: EMDB-29291, PDB-8fmb: |
由来 |
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キーワード | VIRAL PROTEIN / Nodavirus RNA replication and RNA capping complex / Dodecamer ring / Outer mitochondrial membrane protein complex / Nodavirus RNA replication protein A polymerase domain / RNA dependent RNA polymerase |