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-Structure paper
タイトル | Structural snapshots of actively translating human ribosomes. |
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ジャーナル・号・ページ | Cell, Vol. 161, Issue 4, Page 845-857, Year 2015 |
掲載日 | 2015年5月7日 |
著者 | Elmar Behrmann / Justus Loerke / Tatyana V Budkevich / Kaori Yamamoto / Andrea Schmidt / Pawel A Penczek / Matthijn R Vos / Jörg Bürger / Thorsten Mielke / Patrick Scheerer / Christian M T Spahn / |
PubMed 要旨 | Macromolecular machines, such as the ribosome, undergo large-scale conformational changes during their functional cycles. Although their mode of action is often compared to that of mechanical ...Macromolecular machines, such as the ribosome, undergo large-scale conformational changes during their functional cycles. Although their mode of action is often compared to that of mechanical machines, a crucial difference is that, at the molecular dimension, thermodynamic effects dominate functional cycles, with proteins fluctuating stochastically between functional states defined by energetic minima on an energy landscape. Here, we have used cryo-electron microscopy to image ex-vivo-derived human polysomes as a source of actively translating ribosomes. Multiparticle refinement and 3D variability analysis allowed us to visualize a variety of native translation intermediates. Significantly populated states include not only elongation cycle intermediates in pre- and post-translocational states, but also eEF1A-containing decoding and termination/recycling complexes. Focusing on the post-translocational state, we extended this assessment to the single-residue level, uncovering striking details of ribosome-ligand interactions and identifying both static and functionally important dynamic elements. |
リンク | Cell / PubMed:25957688 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.5 - 9.5 Å |
構造データ | EMDB-2875, PDB-5aj0: EMDB-2902: EMDB-2903: EMDB-2904: EMDB-2905: EMDB-2906: EMDB-2907: EMDB-2908: EMDB-2909: EMDB-2910: EMDB-2911: |
化合物 | ChemComp-MG: ChemComp-ZN: |
由来 |
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キーワード | RIBOSOME / MAMMALIAN RIBOSOME / TRANSLATION / POLYSOME / CRYO ELECTRON MICROSCOPY / ELONGATION CYCLE |