+検索条件
-Structure paper
タイトル | Architecture of Human IgM in Complex with P. falciparum Erythrocyte Membrane Protein 1. |
---|---|
ジャーナル・号・ページ | Cell Rep, Vol. 14, Issue 4, Page 723-736, Year 2016 |
掲載日 | 2016年2月2日 |
著者 | Reetesh Raj Akhouri / Suchi Goel / Hirotoshi Furusho / Ulf Skoglund / Mats Wahlgren / |
PubMed 要旨 | Plasmodium falciparum virulence is associated with sequestration of infected erythrocytes. Microvascular binding mediated by PfEMP1 in complex with non-immune immunoglobulin M (IgM) is common among ...Plasmodium falciparum virulence is associated with sequestration of infected erythrocytes. Microvascular binding mediated by PfEMP1 in complex with non-immune immunoglobulin M (IgM) is common among parasites that cause both severe childhood malaria and pregnancy-associated malaria. Here, we present cryo-molecular electron tomography structures of human IgM, PfEMP1 and their complex. Three-dimensional reconstructions of IgM reveal that it has a dome-like core, randomly oriented Fab2s units, and the overall shape of a turtle. PfEMP1 is a C- shaped molecule with a flexible N terminus followed by an arc-shaped backbone and a bulky C terminus that interacts with IgM. Our data demonstrate that the PfEMP1 binding pockets on IgM overlap with those of C1q, and the bulkiness of PfEMP1 limits the capacity of IgM to interact with PfEMP1. We suggest that P. falciparum exploits IgM to cluster PfEMP1 into an organized matrix to augment its affinity to host cell receptors. |
リンク | Cell Rep / PubMed:26776517 |
手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
解像度 | 20.0 - 25.0 Å |
構造データ | EMDB-2882: EMDB-2883: EMDB-2884: EMDB-2885: EMDB-2886: EMDB-2887: EMDB-2888: EMDB-2889: EMDB-2890: EMDB-2891: EMDB-2892: EMDB-2893: EMDB-2894: EMDB-2895: EMDB-6554: |
由来 |
|