EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ligand-mediated Structural Dynamics of a Mammalian Pancreatic K Channel.
ジャーナル・号・ページ	J Mol Biol, Vol. 434, Issue 19, Page 167789, Year 2022
掲載日	2022年8月11日
著者	Min Woo Sung / Camden M Driggers / Barmak Mostofian / John D Russo / Bruce L Patton / Daniel M Zuckerman / Show-Ling Shyng /
PubMed 要旨	Regulation of pancreatic K channels involves orchestrated interactions of their subunits, Kir6.2 and SUR1, and ligands. Previously we reported K channel cryo-EM structures in the presence and absence ...Regulation of pancreatic K channels involves orchestrated interactions of their subunits, Kir6.2 and SUR1, and ligands. Previously we reported K channel cryo-EM structures in the presence and absence of pharmacological inhibitors and ATP, focusing on the mechanisms by which inhibitors act as pharmacological chaperones of K channels (Martin et al., 2019). Here we analyzed the same cryo-EM datasets with a focus on channel conformational dynamics to elucidate structural correlates pertinent to ligand interactions and channel gating. We found pharmacological inhibitors and ATP enrich a channel conformation in which the Kir6.2 cytoplasmic domain is closely associated with the transmembrane domain, while depleting one where the Kir6.2 cytoplasmic domain is extended away into the cytoplasm. This conformational change remodels a network of intra- and inter-subunit interactions as well as the ATP and PIP binding pockets. The structures resolved key contacts between the distal N-terminus of Kir6.2 and SUR1's ABC module involving residues implicated in channel function and showed a SUR1 residue, K134, participates in PIP binding. Molecular dynamics simulations revealed two Kir6.2 residues, K39 and R54, that mediate both ATP and PIP binding, suggesting a mechanism for competitive gating by ATP and PIP.
リンク	J Mol Biol / PubMed:35964676 / PubMed Central
手法	EM (単粒子)
解像度	3.41 - 7.4 Å
構造データ	26193 7tys EMDB-26193, PDB-7tys: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and repaglinide with Kir6.2-CTD in the up conformation 手法: EM (単粒子) / 解像度: 3.41 Å 26194 7tyt EMDB-26194, PDB-7tyt: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and repaglinide with Kir6.2-CTD in the down conformation 手法: EM (単粒子) / 解像度: 3.6 Å 26299 7u1e EMDB-26299, PDB-7u1e: CryoEM structure of the pancreatic ATP-sensitive potassium channel bound to ATP with Kir6.2-CTD in the down conformation 手法: EM (単粒子) / 解像度: 4.52 Å 26303 7u1q EMDB-26303, PDB-7u1q: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and repaglinide with SUR1-in conformation 手法: EM (単粒子) / 解像度: 3.9 Å 26304 7u1s EMDB-26304, PDB-7u1s: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and repaglinide with SUR1-out conformation 手法: EM (単粒子) / 解像度: 3.8 Å 26307 7u24 EMDB-26307, PDB-7u24: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and glibenclamide with Kir6.2-CTD in the up conformation 手法: EM (単粒子) / 解像度: 3.58 Å 26308 7u6y EMDB-26308: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and glibenclamide with Kir6.2-CTD in the down conformation PDB-7u6y: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel in the presence of glibenclamide and ATP with Kir6.2-CTD in the down conformation 手法: EM (単粒子) / 解像度: 6.8 Å 26309 7u7m EMDB-26309: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP and in the presence of carbamazepine with Kir6.2-CTD in the up conformation PDB-7u7m: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel in the presence of carbamazepine and ATP with Kir6.2-CTD in the up conformation 手法: EM (単粒子) / 解像度: 5.2 Å 26312 7uaa EMDB-26312: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel bound to ATP with Kir6.2-CTD in the up conformation PDB-7uaa: CryoEM structure of the pancreatic ATP-sensitive potassium channel in the ATP-bound state with Kir6.2-CTD in the up conformation 手法: EM (単粒子) / 解像度: 5.7 Å 26320 7uqr EMDB-26320, PDB-7uqr: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel in the apo form with Kir6.2-CTD in the down conformation 手法: EM (単粒子) 26321 7u2x EMDB-26321, PDB-7u2x: Cryo-EM structure of the pancreatic ATP-sensitive potassium channel in the presence of carbamazepine and ATP with Kir6.2-CTD in the down conformation 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-K: Unknown entry / カリウムカチオン ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM / POホスファチジルコリン ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / O-(1-O,2-O-ジステアロイル-L-グリセロ-3-ホスホ)セリン / ホスファチジルセリン ChemComp-65I: (9R,12R)-15-amino-12-hydroxy-6,12-dioxo-7,11,13-trioxa-12lambda~5~-phosphapentadecan-9-yl undecanoate ChemComp-BJX: Repaglinide / レパグリニド / 薬剤YM / レパグリニド ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-GBM: 5-chloro-N-(2-{4-[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzamide / グリベンクラミド / 薬剤*YM / グリベンクラミド
由来	cricetus cricetus (クロハラハムスクー) rattus norvegicus (ドブネズミ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / KATP channel (ATP感受性カリウムチャネル) / SUR1 / Kir6.2 / repaglinide (レパグリニド) / RPG / sulfonylurea receptor / potassium transport / glibenclamide (グリベンクラミド) / GBC / GBM / metabolic sensor