EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and biochemical rationale for enhanced spike protein fitness in delta and kappa SARS-CoV-2 variants.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 742, Year 2022
掲載日	2022年2月8日
著者	James W Saville / Dhiraj Mannar / Xing Zhu / Shanti S Srivastava / Alison M Berezuk / Jean-Philippe Demers / Steven Zhou / Katharine S Tuttle / Inna Sekirov / Andrew Kim / Wei Li / Dimiter S Dimitrov / Sriram Subramaniam /
PubMed 要旨	The Delta and Kappa variants of SARS-CoV-2 co-emerged in India in late 2020, with the Delta variant underlying the resurgence of COVID-19, even in countries with high vaccination rates. In this ...The Delta and Kappa variants of SARS-CoV-2 co-emerged in India in late 2020, with the Delta variant underlying the resurgence of COVID-19, even in countries with high vaccination rates. In this study, we assess structural and biochemical aspects of viral fitness for these two variants using cryo-electron microscopy (cryo-EM), ACE2-binding and antibody neutralization analyses. Both variants demonstrate escape of antibodies targeting the N-terminal domain, an important immune hotspot for neutralizing epitopes. Compared to wild-type and Kappa lineages, Delta variant spike proteins show modest increase in ACE2 affinity, likely due to enhanced electrostatic complementarity at the RBD-ACE2 interface, which we characterize by cryo-EM. Unexpectedly, Kappa variant spike trimers form a structural head-to-head dimer-of-trimers assembly, which we demonstrate is a result of the E484Q mutation and with unknown biological implications. The combination of increased antibody escape and enhanced ACE2 binding provides an explanation, in part, for the rapid global dominance of the Delta variant.
リンク	Nat Commun / PubMed:35136050 / PubMed Central
手法	EM (単粒子)
解像度	2.25 - 3.62 Å
構造データ	25853 7tew EMDB-25853, PDB-7tew: Cryo-EM structure of SARS-CoV-2 Delta (B.1.617.2) spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.52 Å 25854 7tex EMDB-25854, PDB-7tex: Cryo-EM structure of SARS-CoV-2 Delta (B.1.617.2) spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 3.27 Å 25855 7tey EMDB-25855, PDB-7tey: Cryo-EM structure of SARS-CoV-2 Delta (B.1.617.2) spike protein 手法: EM (単粒子) / 解像度: 2.25 Å 25856 7tez EMDB-25856, PDB-7tez: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.27 Å 25857 7tf0 EMDB-25857, PDB-7tf0: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 3.02 Å 25858 7tf1 EMDB-25858, PDB-7tf1: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) Q484I spike protein (focused refinement of RBD) 手法: EM (単粒子) / 解像度: 3.57 Å 25859 7tf2 EMDB-25859, PDB-7tf2: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) Q484I spike protein 手法: EM (単粒子) / 解像度: 3.62 Å 25860 7tf3 EMDB-25860, PDB-7tf3: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) Q484A spike protein 手法: EM (単粒子) / 解像度: 2.25 Å 25861 7tf4 EMDB-25861, PDB-7tf4: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) spike protein (focused refinement of RBD) 手法: EM (単粒子) / 解像度: 3.01 Å 25862 7tf5 EMDB-25862, PDB-7tf5: Cryo-EM structure of SARS-CoV-2 Kappa (B.1.617.1) spike protein 手法: EM (単粒子) / 解像度: 3.16 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN / SARS-CoV-2 / glycoprotein / fusion protein / ACE2