EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular structure of an open human K channel.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 48, Year 2021
掲載日	2021年11月30日
著者	Chen Zhao / Roderick MacKinnon /
PubMed 要旨	K channels are metabolic sensors that translate intracellular ATP/ADP balance into membrane excitability. The molecular composition of K includes an inward-rectifier potassium channel (Kir) and an ...K channels are metabolic sensors that translate intracellular ATP/ADP balance into membrane excitability. The molecular composition of K includes an inward-rectifier potassium channel (Kir) and an ABC transporter-like sulfonylurea receptor (SUR). Although structures of K have been determined in many conformations, in all cases, the pore in Kir is closed. Here, we describe human pancreatic K (hK) structures with an open pore at 3.1- to 4.0-Å resolution using single-particle cryo-electron microscopy (cryo-EM). Pore opening is associated with coordinated structural changes within the ATP-binding site and the channel gate in Kir. Conformational changes in SUR are also observed, resulting in an area reduction of contact surfaces between SUR and Kir. We also observe that pancreatic hK exhibits the unique (among inward-rectifier channels) property of PIP-independent opening, which appears to be correlated with a docked cytoplasmic domain in the absence of PIP.
リンク	Proc Natl Acad Sci U S A / PubMed:34815345 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.0 Å
構造データ	24839 7s5t EMDB-24839, PDB-7s5t: Human KATP channel in open conformation, focused on Kir (C166S G334D double mutant) and SUR TMD0 手法: EM (単粒子) / 解像度: 3.1 Å 24840 7s5v EMDB-24840, PDB-7s5v: Human KATP channel in open conformation, focused on SUR 手法: EM (単粒子) / 解像度: 3.3 Å 24842 7s5x EMDB-24842, PDB-7s5x: Human KATP channel in open conformation, focused on Kir and one SUR, position 1 手法: EM (単粒子) / 解像度: 3.7 Å 24843 7s5y EMDB-24843, PDB-7s5y: Human KATP channel in open conformation, focused on Kir and one SUR, position 2 手法: EM (単粒子) / 解像度: 3.9 Å 24844 7s5z EMDB-24844, PDB-7s5z: Human KATP channel in open conformation, focused on Kir and one SUR, position 3 手法: EM (単粒子) / 解像度: 3.9 Å 24845 7s60 EMDB-24845, PDB-7s60: Human KATP channel in open conformation, focused on Kir and one SUR, position 4 手法: EM (単粒子) / 解像度: 3.7 Å 24846 7s61 EMDB-24846, PDB-7s61: Human KATP channel in open conformation, focused on Kir and one SUR, position 5 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / ion channel (イオンチャネル) / KATP (ATP感受性カリウムチャネル) / ATP-sensitive potassium channel (ATP感受性カリウムチャネル)