EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	TMEM16 scramblases thin the membrane to enable lipid scrambling.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 2604, Year 2022
掲載日	2022年5月11日
著者	Maria E Falzone / Zhang Feng / Omar E Alvarenga / Yangang Pan / ByoungCheol Lee / Xiaolu Cheng / Eva Fortea / Simon Scheuring / Alessio Accardi /
PubMed 要旨	TMEM16 scramblases dissipate the plasma membrane lipid asymmetry to activate multiple eukaryotic cellular pathways. Scrambling was proposed to occur with lipid headgroups moving between leaflets ...TMEM16 scramblases dissipate the plasma membrane lipid asymmetry to activate multiple eukaryotic cellular pathways. Scrambling was proposed to occur with lipid headgroups moving between leaflets through a membrane-spanning hydrophilic groove. Direct information on lipid-groove interactions is lacking. We report the 2.3 Å resolution cryogenic electron microscopy structure of the nanodisc-reconstituted Ca-bound afTMEM16 scramblase showing how rearrangement of individual lipids at the open pathway results in pronounced membrane thinning. Only the groove's intracellular vestibule contacts lipids, and mutagenesis suggests scrambling does not require specific protein-lipid interactions with the extracellular vestibule. We find scrambling can occur outside a closed groove in thinner membranes and is inhibited in thicker membranes, despite an open pathway. Our results show afTMEM16 thins the membrane to enable scrambling and that an open hydrophilic pathway is not a structural requirement to allow rapid transbilayer movement of lipids. This mechanism could be extended to other scramblases lacking a hydrophilic groove.
リンク	Nat Commun / PubMed:35562175 / PubMed Central
手法	EM (単粒子)
解像度	2.28 - 3.5 Å
構造データ	24717 7rwj EMDB-24717, PDB-7rwj: afTMEM16 in C22 lipid nanodiscs with MSP2N2 scaffold protein in the presnece of Ca2+ 手法: EM (単粒子) / 解像度: 3.5 Å 24722 7rx2 EMDB-24722, PDB-7rx2: afTMEM16 in C22 lipid nanodiscs with MSP1E3 scaffold protein in the presnece of Ca2+ 手法: EM (単粒子) / 解像度: 2.7 Å 24723 7rx3 EMDB-24723, PDB-7rx3: afTMEM16 in C14 lipid nanodiscs with MSP1E3 scaffold protein in the absence of Ca2+ 手法: EM (単粒子) / 解像度: 3.3 Å 24726 7rxa EMDB-24726, PDB-7rxa: afTMEM16 DE/AA mutant in C14 lipid nanodiscs in the presence of Ca2+ 手法: EM (単粒子) / 解像度: 3.08 Å 24727 7rxb EMDB-24727, PDB-7rxb: afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+ 手法: EM (単粒子) / 解像度: 3.07 Å 24730 7rxg EMDB-24730, PDB-7rxg: afTMEM16 in C18 lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+, full dimer 手法: EM (単粒子) / 解像度: 2.28 Å 24731 7rxh EMDB-24731, PDB-7rxh: afTMEM16 in C18 lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+, monomer with extra lipids 手法: EM (単粒子) / 解像度: 2.3 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-PGW: (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl / POPG / リン脂質YM ChemComp-HOH*: WATER
由来	Aspergillus fumigatus (カビ) neosartorya fumigata (strain atcc mya-4609 / af293 / cbs 101355 / fgsc a1100) (カビ) neosartorya fumigata (strain cea10 / cbs 144.89 / fgsc a1163) (カビ)
キーワード	LIPID TRANSPORT / TMEM16 / lipid scrambling