EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and superorganization of acetylcholine receptor-rapsyn complexes.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 110, Issue 26, Page 10622-10627, Year 2013
掲載日	2013年6月25日
著者	Benoît Zuber / Nigel Unwin /
PubMed 要旨	The scaffolding protein at the neuromuscular junction, rapsyn, enables clustering of nicotinic acetylcholine receptors in high concentration and is critical for muscle function. Patients with ...The scaffolding protein at the neuromuscular junction, rapsyn, enables clustering of nicotinic acetylcholine receptors in high concentration and is critical for muscle function. Patients with insufficient receptor clustering suffer from muscle weakness. However, the detailed organization of the receptor-rapsyn network is poorly understood: it is unclear whether rapsyn first forms a wide meshwork to which receptors can subsequently dock or whether it only forms short bridges linking receptors together to make a large cluster. Furthermore, the number of rapsyn-binding sites per receptor (a heteropentamer) has been controversial. Here, we show by cryoelectron tomography and subtomogram averaging of Torpedo postsynaptic membrane that receptors are connected by up to three rapsyn bridges, the minimum number required to form a 2D network. Half of the receptors belong to rapsyn-connected groups comprising between two and fourteen receptors. Our results provide a structural basis for explaining the stability and low diffusion of receptors within clusters.
リンク	Proc Natl Acad Sci U S A / PubMed:23754381 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	40 - 50 Å
構造データ	2376 4bog EMDB-2376, PDB-4bog: The structure and super-organization of acetylcholine receptor-rapsyn complexes 手法: EM (サブトモグラム平均) 2377 4boi EMDB-2377: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class A PDB-4boi: The structure and super-organization of acetylcholine receptor-rapsyn complexes class A 手法: EM (サブトモグラム平均) / 解像度: 41.0 Å 2378 4bon EMDB-2378: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class B PDB-4bon: The structure and super-organization of acetylcholine receptor-rapsyn complexes class B 手法: EM (サブトモグラム平均) / 解像度: 41.0 Å 2381 4boo EMDB-2381: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class C PDB-4boo: The structure and super-organization of acetylcholine receptor-rapsyn complexes class C 手法: EM (サブトモグラム平均) / 解像度: 42.0 Å 2382 4bor EMDB-2382: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class D PDB-4bor: The structure and super-organization of acetylcholine receptor-rapsyn complexes class D 手法: EM (サブトモグラム平均) / 解像度: 42.0 Å 2383 4bot EMDB-2383: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class E PDB-4bot: The structure and super-organization of acetylcholine receptor- rapsyn complexes class E 手法: EM (サブトモグラム平均) / 解像度: 42.0 Å
由来	torpedo marmorata (エイ)
キーワード	TRANSPORT PROTEIN / CLUSTERING / SYNAPSE / NEUROMUSCULAR JUNCTION / NICOTINIC / RAPSYN / 43K / ELECTRIC ORGAN / NEUROTRANSMITTER RECEPTOR / RECEPTOR