EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.
ジャーナル・号・ページ	Cell, Vol. 184, Issue 11, Page 2955-2972.e25, Year 2021
掲載日	2021年5月27日
著者	Wilton B Williams / R Ryan Meyerhoff / R J Edwards / Hui Li / Kartik Manne / Nathan I Nicely / Rory Henderson / Ye Zhou / Katarzyna Janowska / Katayoun Mansouri / Sophie Gobeil / Tyler Evangelous / Bhavna Hora / Madison Berry / A Yousef Abuahmad / Jordan Sprenz / Margaret Deyton / Victoria Stalls / Megan Kopp / Allen L Hsu / Mario J Borgnia / Guillaume B E Stewart-Jones / Matthew S Lee / Naomi Bronkema / M Anthony Moody / Kevin Wiehe / Todd Bradley / S Munir Alam / Robert J Parks / Andrew Foulger / Thomas Oguin / Gregory D Sempowski / Mattia Bonsignori / Celia C LaBranche / David C Montefiori / Michael Seaman / Sampa Santra / John Perfect / Joseph R Francica / Geoffrey M Lynn / Baptiste Aussedat / William E Walkowicz / Richard Laga / Garnett Kelsoe / Kevin O Saunders / Daniela Fera / Peter D Kwong / Robert A Seder / Alberto Bartesaghi / George M Shaw / Priyamvada Acharya / Barton F Haynes /
PubMed 要旨	Natural antibodies (Abs) can target host glycans on the surface of pathogens. We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) ...Natural antibodies (Abs) can target host glycans on the surface of pathogens. We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen. 2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (V) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization. Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without V-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques. FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike. FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans. Moreover, FDG precursors were predominately mutated IgMIgDCD27, thus suggesting that they originated from a pool of antigen-experienced IgM or marginal zone B cells.
リンク	Cell / PubMed:34019795 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.61 - 7.0 Å
構造データ	23094 7l02 EMDB-23094, PDB-7l02: Cryo-EM structure of SARS-CoV-2 2P S ectodomain bound to one copy of domain-swapped antibody 2G12 手法: EM (単粒子) / 解像度: 3.2 Å 23095 7l06 EMDB-23095, PDB-7l06: Cryo-EM structure of SARS-CoV-2 2P S ectodomain bound to two copies of domain-swapped antibody 2G12 手法: EM (単粒子) / 解像度: 3.3 Å 23097 7l09 EMDB-23097, PDB-7l09: Cryo-EM structure of SARS-CoV-2 2P S ectodomain bound domain-swapped antibody 2G12 from masked 3D refinement 手法: EM (単粒子) / 解像度: 3.1 Å 23124 7l6o EMDB-23124: Cryo-electron microcospy reconstruction of CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 HIV Env PDB-7l6o: Cryo-EM structure of HIV-1 Env CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 手法: EM (単粒子) / 解像度: 3.9 Å 23145 7l6m EMDB-23145: Cryo-electron microscopy reconstruction of locally refined antibody DH898.1 Fab-dimer PDB-7l6m: Cryo-EM structure of DH898.1 Fab-dimer from local refinement of the Fab-dimer bound near the CD4 binding site of HIV-1 Env CH848 SOSIP trimer 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-23152: Cryo-electron microscopy reconstruction of antibody DH898.1 Fab-dimer bound to glycans 332, 392, and 396 of HIV Env CH848 10.17 SOSIP trimer 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-23153: Cryo-electron microscopy local refinement of antibody DH898.1 Fab-dimer bound to glycans 332, 392, and 396 of HIV Env CH848 10.17 SOSIP trimer 手法: EM (単粒子) / 解像度: 7.0 Å 23518 7lu9 EMDB-23518: Cryo-EM map of DH851.3 bound to HIV-1 CH505 Env PDB-7lu9: Cryo-EM structure of DH851.3 bound to HIV-1 CH505 Env 手法: EM (単粒子) / 解像度: 5.6 Å 23519 7lua EMDB-23519: Cryo-EM map of DH898.1 Fab-dimer bound near the CD4 binding site of HIV-1 Env CH848 SOSIP trimer PDB-7lua: Cryo-EM structure of DH898.1 Fab-dimer bound near the CD4 binding site of HIV-1 Env CH848 SOSIP trimer 手法: EM (単粒子) / 解像度: 4.7 Å PDB-6vtu: DH717.1 Fab monomer in complex with man9 glycan 手法: X-RAY DIFFRACTION / 解像度: 2.61 Å PDB-6xrj: Crystal structure of the disulfide linked DH717.1 Fab dimer, derived from a macaque HIV-1 vaccine-induced Env glycan-reactive neutralizing antibody B cell lineage 手法: X-RAY DIFFRACTION / 解像度: 3.15 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-HOH: WATER / 水 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	Severe acute respiratory syndrome-related coronavirus (SARSコロナウイルス) severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト) human immunodeficiency virus 1 (ヒト免疫不全ウイルス) macaca mulatta (アカゲザル) simian-human immunodeficiency virus (ヒト免疫不全ウイルス)
キーワード	IMMUNE SYSTEM (免疫系) / FAB FRAGMENT / HIV-1 / ANTIBODY (抗体) / Fab-dimerized / glycan-reactive / antibodies (抗体) / HIV (ヒト免疫不全ウイルス) / VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) / SARS (重症急性呼吸器症候群) / COVID19 (新型コロナウイルス感染症 (2019年)) / SARS-CoV-2 2P S ectodomain / VIRAL PROTEIN-IMMUNE SYSTEM complex SARS-CoV-2 2P S ectodomain (ウイルス性) / DH898.1 / Fab-dimer / glycan-reactive neutralizing antibody / macaque HIV-1 vaccine-induced / B cell lineage / VIRAL PROTEIN (ウイルスタンパク質) / HIV-1 Env