EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and function of the amino acid polymerase cyanophycin synthetase.
ジャーナル・号・ページ	Nat Chem Biol, Vol. 17, Issue 10, Page 1101-1110, Year 2021
掲載日	2021年8月12日
著者	Itai Sharon / Asfarul S Haque / Marcel Grogg / Indrajit Lahiri / Dieter Seebach / Andres E Leschziner / Donald Hilvert / T Martin Schmeing /
PubMed 要旨	Cyanophycin is a natural biopolymer produced by a wide range of bacteria, consisting of a chain of poly-L-Asp residues with L-Arg residues attached to the β-carboxylate sidechains by isopeptide ...Cyanophycin is a natural biopolymer produced by a wide range of bacteria, consisting of a chain of poly-L-Asp residues with L-Arg residues attached to the β-carboxylate sidechains by isopeptide bonds. Cyanophycin is synthesized from ATP, aspartic acid and arginine by a homooligomeric enzyme called cyanophycin synthetase (CphA1). CphA1 has domains that are homologous to glutathione synthetases and muramyl ligases, but no other structural information has been available. Here, we present cryo-electron microscopy and X-ray crystallography structures of cyanophycin synthetases from three different bacteria, including cocomplex structures of CphA1 with ATP and cyanophycin polymer analogs at 2.6 Å resolution. These structures reveal two distinct tetrameric architectures, show the configuration of active sites and polymer-binding regions, indicate dynamic conformational changes and afford insight into catalytic mechanism. Accompanying biochemical interrogation of substrate binding sites, catalytic centers and oligomerization interfaces combine with the structures to provide a holistic understanding of cyanophycin biosynthesis.
リンク	Nat Chem Biol / PubMed:34385683
手法	EM (単粒子) / X線回折
解像度	2.6 - 4.4 Å
構造データ	23311 7lg5 EMDB-23311, PDB-7lg5: Synechocystis sp. UTEX2470 Cyanophycin synthetase 1 with ATP 手法: EM (単粒子) / 解像度: 2.63 Å 23325 7lgj EMDB-23325, PDB-7lgj: Cyanophycin synthetase 1 from Synechocystis sp. UTEX2470 with ADPCP and 8x(Asp-Arg)-NH2 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-23326: Cyanophycin synthetase 1 from Synechocystis sp. UTEX2470 with ATP and 16x(Asp-Arg) 手法: EM (単粒子) / 解像度: 2.6 Å 23327 7lgm EMDB-23327, PDB-7lgm: Cyanophycin synthetase from A. baylyi DSM587 with ATP 手法: EM (単粒子) / 解像度: 4.4 Å 23328 7lgq EMDB-23328, PDB-7lgq: Cyanophycin synthetase 1 from Synechocystis sp. UTEX2470 with ATP and 8x(Asp-Arg)-Asn 手法: EM (単粒子) / 解像度: 2.7 Å PDB-7lgn: Cyanophycin synthetase 1 from T. morbirosei 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / β,γ-メチレンATP / AMP-PCP, エネルギー貯蔵分子類似体YM
由来	Synechocystis sp. PCC 6714 (バクテリア) synechocystis sp. (strain pcc 6714) (バクテリア) synthetic construct (人工物) Acinetobacter baylyi ADP1 (バクテリア) acinetobacter baylyi (strain atcc 33305 / bd413 / adp1) (バクテリア) tatumella morbirosei (バクテリア)
キーワード	LIGASE / cyanophycin / CphA1 / ATP grasp / CphA / ATP-grasp / enzyme