EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone system.
ジャーナル・号・ページ	Cell Rep, Vol. 35, Issue 8, Page 109166, Year 2021
掲載日	2021年5月25日
著者	Yanting Yin / Xiang Feng / Hongjun Yu / Allison Fay / Amanda Kovach / Michael S Glickman / Huilin Li /
PubMed 要旨	The M. tuberculosis (Mtb) ClpB is a protein disaggregase that helps to rejuvenate the bacterial cell. DnaK is a protein foldase that can function alone, but it can also bind to the ClpB hexamer to ...The M. tuberculosis (Mtb) ClpB is a protein disaggregase that helps to rejuvenate the bacterial cell. DnaK is a protein foldase that can function alone, but it can also bind to the ClpB hexamer to physically couple protein disaggregation with protein refolding, although the molecular mechanism is not well understood. Here, we report the cryo-EM analysis of the Mtb ClpB-DnaK bi-chaperone in the presence of ATPγS and a protein substrate. We observe three ClpB conformations in the presence of DnaK, identify a conserved TGIP loop linking the oligonucleotide/oligosaccharide-binding domain and the nucleotide-binding domain that is important for ClpB function, derive the interface between the regulatory middle domain of the ClpB and the DnaK nucleotide-binding domain, and find that DnaK binding stabilizes, but does not bend or tilt, the ClpB middle domain. We propose a model for the synergistic actions of aggregate dissolution and refolding by the Mtb ClpB-DnaK bi-chaperone system.
リンク	Cell Rep / PubMed:34038719 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 7.0 Å
構造データ	21553 6w6e EMDB-21553: Cryo-EM map of the Mycobacterium tuberculosis ClpB disaggregase hexamer with a locally refined ClpB middle domain and a DnaK nucleotide binding domain PDB-6w6e: The Mycobacterium tuberculosis ClpB disaggregase hexamer structure with a locally refined ClpB middle domain and a DnaK nucleotide binding domain 手法: EM (単粒子) / 解像度: 3.7 Å 21554 6w6g EMDB-21554: Cryo-EM map of the Mycobacterium tuberculosis ClpB disaggregase hexamer in conformation I in the presence of DnaK chaperone and a model substrate PDB-6w6g: The Mycobacterium tuberculosis ClpB disaggregase hexamer structure in conformation I in the presence of DnaK chaperone and a model substrate 手法: EM (単粒子) / 解像度: 3.1 Å 21555 6w6h EMDB-21555: Cryo-EM map of the Mycobacterium tuberculosis ClpB disaggregase hexamer in conformation II in the presence of DnaK chaperone and a model substrate PDB-6w6h: The Mycobacterium tuberculosis ClpB disaggregase hexamer structure in conformation II in the presence of DnaK chaperone and a model substrate 手法: EM (単粒子) / 解像度: 3.3 Å 21556 6w6i EMDB-21556: Cryo-EM map of the Mycobacterium tuberculosis ClpB disaggregase hexamer in conformation T in the presence of DnaK chaperone and a model substrate PDB-6w6i: The Mycobacterium tuberculosis ClpB disaggregase hexamer structure in conformation T in the presence of DnaK chaperone and a model substrate 手法: EM (単粒子) / 解像度: 3.5 Å 21557 6w6j EMDB-21557: Cryo-EM map of the Mycobacterium tuberculosis ClpB disaggregase hexamer with a locally refined N-terminal domain in the presence of DnaK chaperone and a model substrate PDB-6w6j: The Mycobacterium tuberculosis ClpB disaggregase hexamer structure with a locally refined N-terminal domain in the presence of DnaK chaperone and a model substrate 手法: EM (単粒子) / 解像度: 3.2 Å 23206 7l6n EMDB-23206: CryoEM map of the Mycobacterium tuberculosis ClpB disaggregase hexamer with three locally refined ClpB middle domains and three DnaK nucleotide binding domains PDB-7l6n: The Mycobacterium tuberculosis ClpB disaggregase hexamer structure with three locally refined ClpB middle domains and three DnaK nucleotide binding domains 手法: EM (単粒子) / 解像度: 7.0 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	mycobacterium tuberculosis (結核菌)
キーワード	CHAPERONE / ClpB-DnaK complex / Disaggregate / Unfold / Refold / ClpB/DnaK / protein aggregates / Refold protein