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Yorodumi- PDB-7l6n: The Mycobacterium tuberculosis ClpB disaggregase hexamer structur... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7l6n | ||||||
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Title | The Mycobacterium tuberculosis ClpB disaggregase hexamer structure with three locally refined ClpB middle domains and three DnaK nucleotide binding domains | ||||||
Components |
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Keywords | CHAPERONE / ClpB-DnaK complex / protein aggregates / Unfold / Refold | ||||||
Function / homology | Function and homology information ATP-dependent protein folding chaperone / unfolded protein binding / response to heat / protein refolding / hydrolase activity / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / Resolution: 7 Å | ||||||
Authors | Yin, Y.Y. / Feng, X. / Li, H. | ||||||
Funding support | United States, 1items
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Citation | Journal: Cell Rep / Year: 2021 Title: Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone system. Authors: Yanting Yin / Xiang Feng / Hongjun Yu / Allison Fay / Amanda Kovach / Michael S Glickman / Huilin Li / Abstract: The M. tuberculosis (Mtb) ClpB is a protein disaggregase that helps to rejuvenate the bacterial cell. DnaK is a protein foldase that can function alone, but it can also bind to the ClpB hexamer to ...The M. tuberculosis (Mtb) ClpB is a protein disaggregase that helps to rejuvenate the bacterial cell. DnaK is a protein foldase that can function alone, but it can also bind to the ClpB hexamer to physically couple protein disaggregation with protein refolding, although the molecular mechanism is not well understood. Here, we report the cryo-EM analysis of the Mtb ClpB-DnaK bi-chaperone in the presence of ATPγS and a protein substrate. We observe three ClpB conformations in the presence of DnaK, identify a conserved TGIP loop linking the oligonucleotide/oligosaccharide-binding domain and the nucleotide-binding domain that is important for ClpB function, derive the interface between the regulatory middle domain of the ClpB and the DnaK nucleotide-binding domain, and find that DnaK binding stabilizes, but does not bend or tilt, the ClpB middle domain. We propose a model for the synergistic actions of aggregate dissolution and refolding by the Mtb ClpB-DnaK bi-chaperone system. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7l6n.cif.gz | 831.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7l6n.ent.gz | 676.2 KB | Display | PDB format |
PDBx/mmJSON format | 7l6n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7l6n_validation.pdf.gz | 1.6 MB | Display | wwPDB validaton report |
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Full document | 7l6n_full_validation.pdf.gz | 1.7 MB | Display | |
Data in XML | 7l6n_validation.xml.gz | 136.3 KB | Display | |
Data in CIF | 7l6n_validation.cif.gz | 202.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l6/7l6n ftp://data.pdbj.org/pub/pdb/validation_reports/l6/7l6n | HTTPS FTP |
-Related structure data
Related structure data | 23206MC 6w6eC 6w6gC 6w6hC 6w6iC 6w6jC C: citing same article (ref.) M: map data used to model this data |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 92688.281 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Gene: clpB, MT0397 / Production host: Escherichia coli (E. coli) / References: UniProt: P9WPD0 #2: Protein/peptide | | Mass: 2826.475 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Production host: Escherichia coli (E. coli) #3: Protein | Mass: 66910.680 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) Gene: dnaK_2, dnaK, E5M05_19850, ERS007703_00955, ERS023446_02581, ERS027651_01905, FCN16_12395, SAMEA2682864_01182, SAMEA2683035_02658 Production host: Escherichia coli (E. coli) / References: UniProt: A0A045JRR0 #4: Chemical | ChemComp-AGS / #5: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: ClpB/DnaK complex / Type: COMPLEX / Entity ID: #1-#3 / Source: RECOMBINANT |
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Source (natural) | Organism: Mycobacterium tuberculosis (bacteria) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: NO |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: DIFFRACTION |
Image recording | Electron dose: 2 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
CTF correction | Type: NONE |
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3D reconstruction | Resolution: 7 Å / Resolution method: OTHER / Num. of particles: 45000 Details: Phenix.combine_focused_maps was used to generate the composite map from several local refined maps. The reported resolution is the worst resolution in the local maps. Symmetry type: POINT |