EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Design of multi-scale protein complexes by hierarchical building block fusion.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 2294, Year 2021
掲載日	2021年4月16日
著者	Yang Hsia / Rubul Mout / William Sheffler / Natasha I Edman / Ivan Vulovic / Young-Jun Park / Rachel L Redler / Matthew J Bick / Asim K Bera / Alexis Courbet / Alex Kang / T J Brunette / Una Nattermann / Evelyn Tsai / Ayesha Saleem / Cameron M Chow / Damian Ekiert / Gira Bhabha / David Veesler / David Baker /
PubMed 要旨	A systematic and robust approach to generating complex protein nanomaterials would have broad utility. We develop a hierarchical approach to designing multi-component protein assemblies from two ...A systematic and robust approach to generating complex protein nanomaterials would have broad utility. We develop a hierarchical approach to designing multi-component protein assemblies from two classes of modular building blocks: designed helical repeat proteins (DHRs) and helical bundle oligomers (HBs). We first rigidly fuse DHRs to HBs to generate a large library of oligomeric building blocks. We then generate assemblies with cyclic, dihedral, and point group symmetries from these building blocks using architecture guided rigid helical fusion with new software named WORMS. X-ray crystallography and cryo-electron microscopy characterization show that the hierarchical design approach can accurately generate a wide range of assemblies, including a 43 nm diameter icosahedral nanocage. The computational methods and building block sets described here provide a very general route to de novo designed protein nanomaterials.
リンク	Nat Commun / PubMed:33863889 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.4 - 19.0 Å
構造データ	22305 6xss EMDB-22305, PDB-6xss: CryoEM structure of designed helical fusion protein C4_nat_HFuse-7900 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-22306: CryoEM map of designed helical fusion protein C5_HFuse-3921 手法: EM (単粒子) / 解像度: 8.06 Å EMDB-23168: Dihedral ring D2_Wm-01 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-23169: Dihedral ring D2_Wm-02 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-23170: Dihedral ring D2_Wm-01_trunc 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-23171: Point Group nanocage T_Wm-1606 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-23172: Point Group nanocage I32_Wm-42 手法: EM (単粒子) / 解像度: 9.62 Å EMDB-23173: Crown C5_Crn_HF-12_26 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-23174: Crown C5_Crn_HF-12_26 手法: EM (単粒子) / 解像度: 6.5 Å PDB-6xh5: Hierarchical design of multi-scale protein complexes by combinatorial assembly of oligomeric helical bundle and repeat protein building blocks 手法: X-RAY DIFFRACTION / 解像度: 3.32 Å PDB-6xi6: Hierarchical design of multi-scale protein complexes by combinatorial assembly of oligomeric helical bundle and repeat protein building blocks 手法: X-RAY DIFFRACTION / 解像度: 2.69 Å PDB-6xns: C3_crown-05 手法: X-RAY DIFFRACTION / 解像度: 3.19 Å PDB-6xt4: C3_HD-1069 (1BH-69) - fusion protein of helical bundle and repeat protein 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å
化合物	ChemComp-HOH: WATER
由来	synthetic construct (人工物)
キーワード	DE NOVO PROTEIN / helical fusion / de novo design / HELICAL BUNDLE REPEAT PROTEIN / helical bundle / helical repeat