EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transport mechanism of P4 ATPase phosphatidylcholine flippases.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年12月15日
著者	Lin Bai / Qinglong You / Bhawik K Jain / H Diessel Duan / Amanda Kovach / Todd R Graham / Huilin Li /
PubMed 要旨	The P4 ATPases use ATP hydrolysis to transport large lipid substrates across lipid bilayers. The structures of the endosome- and Golgi-localized phosphatidylserine flippases-such as the yeast Drs2 ...The P4 ATPases use ATP hydrolysis to transport large lipid substrates across lipid bilayers. The structures of the endosome- and Golgi-localized phosphatidylserine flippases-such as the yeast Drs2 and human ATP8A1-have recently been reported. However, a substrate-binding site on the cytosolic side has not been found, and the transport mechanisms of P4 ATPases with other substrates are unknown. Here, we report structures of the Dnf1-Lem3 and Dnf2-Lem3 complexes. We captured substrate phosphatidylcholine molecules on both the exoplasmic and cytosolic sides and found that they have similar structures. Unexpectedly, Lem3 contributes to substrate binding. The conformational transitions of these phosphatidylcholine transporters match those of the phosphatidylserine transporters, suggesting a conserved mechanism among P4 ATPases. Dnf1/Dnf2 have a unique P domain helix-turn-helix insertion that is important for function. Therefore, P4 ATPases may have retained an overall transport mechanism while evolving distinct features for different lipid substrates.
リンク	Elife / PubMed:33320091 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.05 Å
構造データ	23068 7ky5 EMDB-23068, PDB-7ky5: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E2P transition state 手法: EM (単粒子) / 解像度: 3.98 Å 23069 7ky6 EMDB-23069, PDB-7ky6: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the apo E1 state 手法: EM (単粒子) / 解像度: 3.1 Å 23070 7ky7 EMDB-23070, PDB-7ky7: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the apo E1 state 手法: EM (単粒子) / 解像度: 3.08 Å 23071 7ky8 EMDB-23071, PDB-7ky8: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E1-ATP state 手法: EM (単粒子) / 解像度: 3.85 Å 23072 7ky9 EMDB-23072, PDB-7ky9: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E1-ADP state 手法: EM (単粒子) / 解像度: 4.05 Å 23073 7kya EMDB-23073, PDB-7kya: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E2P state 手法: EM (単粒子) / 解像度: 3.5 Å 23074 7kyb EMDB-23074, PDB-7kyb: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the E1-ADP state 手法: EM (単粒子) / 解像度: 3.2 Å 23075 7kyc EMDB-23075, PDB-7kyc: Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the E2P state 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-CLR: CHOLESTEROL / コレステロ-ル / コレステロール ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / β,γ-メチレンATP / AMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス / マンノース ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質*YM / POホスファチジルコリン
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	TRANSLOCASE (輸送酵素) / P4 ATPase / Phosphatidylcholine Flippases