EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Convergence of a common solution for broad ebolavirus neutralization by glycan cap-directed human antibodies.
ジャーナル・号・ページ	Cell Rep, Vol. 35, Issue 2, Page 108984, Year 2021
掲載日	2021年4月13日
著者	Charles D Murin / Pavlo Gilchuk / Philipp A Ilinykh / Kai Huang / Natalia Kuzmina / Xiaoli Shen / Jessica F Bruhn / Aubrey L Bryan / Edgar Davidson / Benjamin J Doranz / Lauren E Williamson / Jeffrey Copps / Tanwee Alkutkar / Andrew I Flyak / Alexander Bukreyev / James E Crowe / Andrew B Ward /
PubMed 要旨	Antibodies that target the glycan cap epitope on the ebolavirus glycoprotein (GP) are common in the adaptive response of survivors. A subset is known to be broadly neutralizing, but the details of ...Antibodies that target the glycan cap epitope on the ebolavirus glycoprotein (GP) are common in the adaptive response of survivors. A subset is known to be broadly neutralizing, but the details of their epitopes and basis for neutralization are not well understood. Here, we present cryoelectron microscopy (cryo-EM) structures of diverse glycan cap antibodies that variably synergize with GP base-binding antibodies. These structures describe a conserved site of vulnerability that anchors the mucin-like domains (MLDs) to the glycan cap, which we call the MLD anchor and cradle. Antibodies that bind to the MLD cradle share common features, including use of IGHV1-69 and IGHJ6 germline genes, which exploit hydrophobic residues and form β-hairpin structures to mimic the MLD anchor, disrupt MLD attachment, destabilize GP quaternary structure, and block cleavage events required for receptor binding. Our results provide a molecular basis for ebolavirus neutralization by broadly reactive glycan cap antibodies.
リンク	Cell Rep / PubMed:33852862 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.002 - 9.9 Å
構造データ	22839 7kej EMDB-22839, PDB-7kej: BDBV-289 bound to EBOV GPdMuc Makona 手法: EM (単粒子) / 解像度: 3.8 Å 22841 7kew EMDB-22841: Bundibugyo virus GP (mucin deleted) bound to antibody Fab BDBV-43 and ADI-15878 PDB-7kew: Bundibugyo virus GP (mucin deleted) bound to antibody Fab BDBV-43 手法: EM (単粒子) / 解像度: 4.16 Å 22842 7kex EMDB-22842, PDB-7kex: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-293 手法: EM (単粒子) / 解像度: 4.25 Å 22847 7kf9 EMDB-22847, PDB-7kf9: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-296 and EBOV-515 手法: EM (単粒子) / 解像度: 4.4 Å 22848 7kfb EMDB-22848: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-442 and EBOV-515 PDB-7kfb: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-442 手法: EM (単粒子) / 解像度: 3.9 Å 22851 7kfe EMDB-22851: Bundibugyo virus GP (mucin deleted) bound to antibody Fab BDBV-329 and EBOV-515 PDB-7kfe: Bundibugyo virus GP (mucin deleted) bound to antibody Fab BDBV-329 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-22852: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-237 and EBOV-515 手法: EM (単粒子) / 解像度: 9.9 Å 22853 7kfh EMDB-22853: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-437 and EBOV-515 PDB-7kfh: Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-437 手法: EM (単粒子) / 解像度: 3.8 Å PDB-7kfg: Antibody Fab BDBV-289 手法: X-RAY DIFFRACTION / 解像度: 3.002 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-PEG: DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル
由来	homo sapiens (ヒト) ebola virus (エボラウイルス) zaire ebolavirus (エボラウイルス) bundibugyo ebolavirus (ウイルス)
キーワード	VIRAL PROTEIN/Immune System / ebolavirus / glycan cap / antibody / broadly neutralizing / filovirus / VIRAL PROTEIN / VIRAL PROTEIN-Immune System complex / IMMUNE SYSTEM