EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of an activated DNA-PK and its implications for NHEJ.
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 4, Page 801-810.e3, Year 2021
掲載日	2021年2月18日
著者	Xuemin Chen / Xiang Xu / Yun Chen / Joyce C Cheung / Huaibin Wang / Jiansen Jiang / Natalia de Val / Tara Fox / Martin Gellert / Wei Yang /
PubMed 要旨	DNA-dependent protein kinase (DNA-PK), like all phosphatidylinositol 3-kinase-related kinases (PIKKs), is composed of conserved FAT and kinase domains (FATKINs) along with solenoid structures made of ...DNA-dependent protein kinase (DNA-PK), like all phosphatidylinositol 3-kinase-related kinases (PIKKs), is composed of conserved FAT and kinase domains (FATKINs) along with solenoid structures made of HEAT repeats. These kinases are activated in response to cellular stress signals, but the mechanisms governing activation and regulation remain unresolved. For DNA-PK, all existing structures represent inactive states with resolution limited to 4.3 Å at best. Here, we report the cryoelectron microscopy (cryo-EM) structures of DNA-PKcs (DNA-PK catalytic subunit) bound to a DNA end or complexed with Ku70/80 and DNA in both inactive and activated forms at resolutions of 3.7 Å overall and 3.2 Å for FATKINs. These structures reveal the sequential transition of DNA-PK from inactive to activated forms. Most notably, activation of the kinase involves previously unknown stretching and twisting within individual solenoid segments and loosens DNA-end binding. This unprecedented structural plasticity of helical repeats may be a general regulatory mechanism of HEAT-repeat proteins.
リンク	Mol Cell / PubMed:33385326 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.21 - 4.3 Å
構造データ	22618 7k0y EMDB-22618, PDB-7k0y: Cryo-EM structure of activated-form DNA-PK (complex VI) 手法: EM (単粒子) / 解像度: 3.7 Å 22619 7k10 EMDB-22619, PDB-7k10: CryoEM structure of activated-form FATKIN domain of DNA-PK 手法: EM (単粒子) / 解像度: 3.3 Å 22620 7k11 EMDB-22620, PDB-7k11: CryoEM structure of inactivated-form FATKIN domain of DNA-PK 手法: EM (単粒子) / 解像度: 3.21 Å 22622 7k19 EMDB-22622, PDB-7k19: CryoEM structure of DNA-PK catalytic subunit complexed with DNA (Complex I) 手法: EM (単粒子) / 解像度: 4.3 Å 22623 7k1b EMDB-22623, PDB-7k1b: CryoEM structure of DNA-PK catalytic subunit complexed with DNA (Complex II) 手法: EM (単粒子) / 解像度: 4.3 Å 22624 7k1j EMDB-22624, PDB-7k1j: CryoEM structure of inactivated-form DNA-PK (Complex III) 手法: EM (単粒子) / 解像度: 3.9 Å 22625 7k1k EMDB-22625, PDB-7k1k: CryoEM structure of inactivated-form DNA-PK (Complex IV) 手法: EM (単粒子) / 解像度: 4.1 Å 22626 7k1n EMDB-22626, PDB-7k1n: CryoEM structure of inactivated-form DNA-PK (Complex V) 手法: EM (単粒子) / 解像度: 3.9 Å PDB-7k17: Re-refined crystal structure of DNA-dependent protein kinase catalytic subunit complexed with Ku80 C-terminal helix 手法: X-RAY DIFFRACTION / 解像度: 4.3 Å
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN/DNA / DNA-dependent Protein Kinase / DNA repair (DNA修復) / NHEJ (非相同末端結合) / V(D)J recombination (V(D)J遺伝子再構成) / DNA BINDING PROTEIN (DNA結合タンパク質) / DNA BINDING PROTEIN-DNA complex / PIKK kinase