EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of transcription-translation coupling.
ジャーナル・号・ページ	Science, Vol. 369, Issue 6509, Page 1359-1365, Year 2020
掲載日	2020年9月11日
著者	Chengyuan Wang / Vadim Molodtsov / Emre Firlar / Jason T Kaelber / Gregor Blaha / Min Su / Richard H Ebright /
PubMed 要旨	In bacteria, transcription and translation are coupled processes in which the movement of RNA polymerase (RNAP)-synthesizing messenger RNA (mRNA) is coordinated with the movement of the first ...In bacteria, transcription and translation are coupled processes in which the movement of RNA polymerase (RNAP)-synthesizing messenger RNA (mRNA) is coordinated with the movement of the first ribosome-translating mRNA. Coupling is modulated by the transcription factors NusG (which is thought to bridge RNAP and the ribosome) and NusA. Here, we report cryo-electron microscopy structures of transcription-translation complexes (TTCs) containing different-length mRNA spacers between RNAP and the ribosome active-center P site. Structures of TTCs containing short spacers show a state incompatible with NusG bridging and NusA binding (TTC-A, previously termed "expressome"). Structures of TTCs containing longer spacers reveal a new state compatible with NusG bridging and NusA binding (TTC-B) and reveal how NusG bridges and NusA binds. We propose that TTC-B mediates NusG- and NusA-dependent transcription-translation coupling.
リンク	Science / PubMed:32820061 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 14.0 Å
構造データ	21386 6vu3 EMDB-21386, PDB-6vu3: Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer 手法: EM (単粒子) / 解像度: 3.7 Å 21468 6vyq EMDB-21468, PDB-6vyq: Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 15 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 3.7 Å 21469 6vyr EMDB-21469, PDB-6vyr: Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 18 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 3.8 Å 21470 6vys EMDB-21470, PDB-6vys: Escherichia coli transcription-translation complex A1 (TTC-A1) containing a 21 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 3.7 Å 21471 6vyt EMDB-21471, PDB-6vyt: Escherichia coli transcription-translation complex A2 (TTC-A2) containing a 15 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 14.0 Å 21472 6vyu EMDB-21472, PDB-6vyu: Escherichia coli transcription-translation complex C2 (TTC-C2) containing a 27 nt long mRNA spacer 手法: EM (単粒子) / 解像度: 7.0 Å 21474 6vyw EMDB-21474, PDB-6vyw: Escherichia coli transcription-translation complex C3 (TTC-C3) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 7.0 Å 21475 6vyx EMDB-21475, PDB-6vyx: Escherichia coli transcription-translation complex C4 (TTC-C4) containing mRNA with a 21 nt long spacer, transcription factor NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 9.9 Å 21476 6vyy EMDB-21476, PDB-6vyy: Escherichia coli transcription-translation complex C5 (TTC-C5) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 9.9 Å 21477 6vyz EMDB-21477, PDB-6vyz: Escherichia coli transcription-translation complex C6 (TTC-C6) containing mRNA with a 21 nt long spacer, NusA, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 9.9 Å 21482 6vz2 EMDB-21482, PDB-6vz2: Escherichia coli transcription-translation complex D1 (TTC-D1) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 10.0 Å 21483 6vz3 EMDB-21483, PDB-6vz3: Escherichia coli transcription-translation complex D2 (TTC-D2) containing mRNA with a 27 nt long spacer 手法: EM (単粒子) / 解像度: 8.9 Å 21485 6vz5 EMDB-21485, PDB-6vz5: Escherichia coli transcription-translation complex D3 (TTC-D3) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 8.9 Å 21486 6vz7 EMDB-21486, PDB-6vz7: Escherichia coli transcription-translation complex C1 (TTC-C1) containing a 27 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 7.0 Å 21494 6vzj EMDB-21494, PDB-6vzj: Escherichia coli transcription-translation complex A1 (TTC-A1) containing mRNA with a 15 nt long spacer, fMet-tRNAs at E-site and P-site, and lacking transcription factor NusG 手法: EM (単粒子) / 解像度: 4.1 Å 22082 6x6t EMDB-22082, PDB-6x6t: Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 3.2 Å 22084 6x7f EMDB-22084, PDB-6x7f: Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 3.5 Å 22087 6x7k EMDB-22087, PDB-6x7k: Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 3.1 Å 22107 6x9q EMDB-22107, PDB-6x9q: Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 4.8 Å 22141 6xdq EMDB-22141, PDB-6xdq: Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site 手法: EM (単粒子) / 解像度: 3.7 Å 22142 6xdr EMDB-22142, PDB-6xdr: Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 4.7 Å 22181 6xgf EMDB-22181, PDB-6xgf: Escherichia coli transcription-translation complex B (TTC-B) containing an 30 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 5.0 Å 22192 6xii EMDB-22192, PDB-6xii: Escherichia coli transcription-translation complex B (TTC-B) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 7.0 Å 22193 6xij EMDB-22193, PDB-6xij: Escherichia coli transcription-translation complex A (TTC-A) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site 手法: EM (単粒子) / 解像度: 8.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	escherichia coli (大腸菌) synthetic construct (人工物) Escherichia coli
キーワード	RIBOSOME / TRANSCRIPTION/TRANSLATION / bacterial coupled transcription-translation complex / TRANSCRIPTION-TRANSLATION complex / Bacterial transcription-translation complex / TRANSCRIPTION / Bacterial transcription-translation coupled complex / Ribosome/transcription/translation / Ribosome-transcription-translation complex / coupled transcription-translation complex