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-Structure paper
タイトル | A pentameric protein ring with novel architecture is required for herpesviral packaging. |
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ジャーナル・号・ページ | Elife, Vol. 10, Year 2021 |
掲載日 | 2021年2月8日 |
著者 | Allison L Didychuk / Stephanie N Gates / Matthew R Gardner / Lisa M Strong / Andreas Martin / Britt A Glaunsinger / |
PubMed 要旨 | Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly ...Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly understood. Here, we present structures of two such accessory factors from the oncogenic herpesviruses Kaposi's sarcoma-associated herpesvirus (KSHV; ORF68) and Epstein-Barr virus (EBV; BFLF1). These homologous proteins form highly similar homopentameric rings with a positively charged central channel that binds double-stranded DNA. Mutation of individual positively charged residues within but not outside the channel ablates DNA binding, and in the context of KSHV infection, these mutants fail to package the viral genome or produce progeny virions. Thus, we propose a model in which ORF68 facilitates the transfer of newly replicated viral genomes to the packaging motor. |
リンク | Elife / PubMed:33554858 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.22 - 3.6 Å |
構造データ | EMDB-22167: EMDB-22168, PDB-6xfa: PDB-6xf9: |
化合物 | ChemComp-ZN: ChemComp-HOH: |
由来 |
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キーワード | VIRAL PROTEIN / viral packaging / viral cleavage |