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-Structure paper
タイトル | The structures of natively assembled clathrin-coated vesicles. |
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ジャーナル・号・ページ | Sci Adv, Vol. 6, Issue 30, Page eaba8397, Year 2020 |
掲載日 | 2020年7月22日 |
著者 | Mohammadreza Paraan / Joshua Mendez / Savanna Sharum / Danielle Kurtin / Huan He / Scott M Stagg / |
PubMed 要旨 | Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy ...Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces. |
リンク | Sci Adv / PubMed:32743076 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 6.3 - 17.9 Å |
構造データ | EMDB-21608: EMDB-21609: EMDB-21610: EMDB-21611: Asymmetric vertex of the clathrin minicoat cage, subparticle refinement of the clathrin layer EMDB-21612: EMDB-21613: EMDB-21614: EMDB-21615: EMDB-21616: |
由来 |
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キーワード | ENDOCYTOSIS / Clathrin coated vesicle / clathrin heavy chain / clathrin light chain / clathrin cage |