EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of redox modulation on chloroplast ATP synthase.
ジャーナル・号・ページ	Commun Biol, Vol. 3, Issue 1, Page 482, Year 2020
掲載日	2020年9月2日
著者	Jay-How Yang / Dewight Williams / Eaazhisai Kandiah / Petra Fromme / Po-Lin Chiu /
PubMed 要旨	In higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the ...In higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the redox modulation, we used single-particle cryo-EM to determine the structures of spinach chloroplast ATP synthase in both reduced and oxidized states. The disulfide linkage of the oxidized γ subunit introduces a torsional constraint to stabilize the two β hairpin structures. Once reduced, free cysteines alleviate this constraint, resulting in a concerted motion of the enzyme complex and a smooth transition between rotary states to facilitate the ATP synthesis. We added an uncompetitive inhibitor, tentoxin, in the reduced sample to limit the flexibility of the enzyme and obtained high-resolution details. Our cryo-EM structures provide mechanistic insight into the redox modulation of the energy regulation activity of chloroplast ATP synthase.
リンク	Commun Biol / PubMed:32879423 / PubMed Central
手法	EM (単粒子)
解像度	3.05 - 7.9 Å
構造データ	21235 6vm1 EMDB-21235, PDB-6vm1: Chloroplast ATP synthase (C3, CF1FO) 手法: EM (単粒子) / 解像度: 7.9 Å 21238 6vm4 EMDB-21238, PDB-6vm4: Chloroplast ATP synthase (C2, CF1FO) 手法: EM (単粒子) / 解像度: 7.08 Å 21239 6vmb EMDB-21239, PDB-6vmb: Chloroplast ATP synthase (C1, CF1FO) 手法: EM (単粒子) / 解像度: 5.23 Å 21240 6vmd EMDB-21240, PDB-6vmd: Chloroplast ATP synthase (C1, CF1) 手法: EM (単粒子) / 解像度: 4.53 Å 21241 6vmg EMDB-21241, PDB-6vmg: Chloroplast ATP synthase (O3, CF1FO) 手法: EM (単粒子) / 解像度: 6.46 Å 21262 6vof EMDB-21262, PDB-6vof: Chloroplast ATP synthase (O2, CF1FO) 手法: EM (単粒子) / 解像度: 4.51 Å 21263 6vog EMDB-21263, PDB-6vog: Chloroplast ATP synthase (O2, CF1) 手法: EM (単粒子) / 解像度: 4.35 Å 21264 6voh EMDB-21264, PDB-6voh: Chloroplast ATP synthase (O1, CF1FO) 手法: EM (単粒子) / 解像度: 4.16 Å 21265 6voi EMDB-21265, PDB-6voi: Chloroplast ATP synthase (O1, CF1) 手法: EM (単粒子) / 解像度: 4.03 Å 21266 6voj EMDB-21266, PDB-6voj: Chloroplast ATP synthase (R3, CF1FO) 手法: EM (単粒子) / 解像度: 4.34 Å 21267 6vok EMDB-21267, PDB-6vok: Chloroplast ATP synthase (R3, CF1) 手法: EM (単粒子) / 解像度: 3.85 Å 21268 6vol EMDB-21268, PDB-6vol: Chloroplast ATP synthase (R2, CF1FO) 手法: EM (単粒子) / 解像度: 4.06 Å 21269 6vom EMDB-21269, PDB-6vom: Chloroplast ATP synthase (R2, CF1) 手法: EM (単粒子) / 解像度: 3.6 Å 21270 6von EMDB-21270, PDB-6von: Chloroplast ATP synthase (R1, CF1FO) 手法: EM (単粒子) / 解像度: 3.35 Å 21271 6voo EMDB-21271, PDB-6voo: Chloroplast ATP synthase (R1, CF1) 手法: EM (単粒子) / 解像度: 3.05 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-TTX: TENTOXIN / toxin*YM / テントキシン
由来	spinacia oleracea (ホウレンソウ) Spinach (ホウレンソウ)
キーワード	PHOTOSYNTHESIS/TRANSLOCASE / CF1FO / ATP synthase (ATP合成酵素) / PHOTOSYNTHESIS (光合成) / PHOTOSYNTHESIS-TRANSLOCASE complex / TRANSLOCASE (輸送酵素)