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-Structure paper
タイトル | Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 27, Issue 2, Page 202-209, Year 2020 |
掲載日 | 2020年2月10日 |
![]() | Wai Kwan Tang / Mario J Borgnia / Allen L Hsu / Lothar Esser / Tara Fox / Natalia de Val / Di Xia / ![]() |
PubMed 要旨 | The mitochondrial membrane-bound AAA protein Bcs1 translocate substrates across the mitochondrial inner membrane without previous unfolding. One substrate of Bcs1 is the iron-sulfur protein (ISP), a ...The mitochondrial membrane-bound AAA protein Bcs1 translocate substrates across the mitochondrial inner membrane without previous unfolding. One substrate of Bcs1 is the iron-sulfur protein (ISP), a subunit of the respiratory Complex III. How Bcs1 translocates ISP across the membrane is unknown. Here we report structures of mouse Bcs1 in two different conformations, representing three nucleotide states. The apo and ADP-bound structures reveal a homo-heptamer and show a large putative substrate-binding cavity accessible to the matrix space. ATP binding drives a contraction of the cavity by concerted motion of the ATPase domains, which could push substrate across the membrane. Our findings shed light on the potential mechanism of translocating folded proteins across a membrane, offer insights into the assembly process of Complex III and allow mapping of human disease-associated mutations onto the Bcs1 structure. |
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手法 | EM (単粒子) / X線回折 |
解像度 | 2.17 - 4.4 Å |
構造データ | EMDB-20808, PDB-6ukp: EMDB-20811, PDB-6uks: ![]() PDB-6u1y: ![]() PDB-6uko: |
化合物 | ![]() ChemComp-ANP: ![]() ChemComp-MG: ![]() ChemComp-HOH: ![]() ChemComp-ADP: ![]() ChemComp-AGS: |
由来 |
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![]() | HYDROLASE / bcs1 / AAA ATPase / CHAPERONE / Rieske / cytochrome bc1 / AAA ATPases / mitochondrial inner membrane |