EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.
ジャーナル・号・ページ	Immunity, Vol. 51, Issue 5, Page 915-929.e7, Year 2019
掲載日	2019年11月19日
著者	Viktoriya Dubrovskaya / Karen Tran / Gabriel Ozorowski / Javier Guenaga / Richard Wilson / Shridhar Bale / Christopher A Cottrell / Hannah L Turner / Gemma Seabright / Sijy O'Dell / Jonathan L Torres / Lifei Yang / Yu Feng / Daniel P Leaman / Néstor Vázquez Bernat / Tyler Liban / Mark Louder / Krisha McKee / Robert T Bailer / Arlette Movsesyan / Nicole A Doria-Rose / Marie Pancera / Gunilla B Karlsson Hedestam / Michael B Zwick / Max Crispin / John R Mascola / Andrew B Ward / Richard T Wyatt /
PubMed 要旨	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge. Most cross-conserved protein determinants are ...The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge. Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface. The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site. Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites. To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting. This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy. This study provides proof-of-principle immunogenicity toward eliciting bNAbs by vaccination.
リンク	Immunity / PubMed:31732167 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.296 - 30.0 Å
構造データ	20259 6p62 EMDB-20259, PDB-6p62: HIV Env BG505 NFL TD+ in complex with antibody E70 fragment antigen binding 手法: EM (単粒子) / 解像度: 3.57 Å 20260 6p65 EMDB-20260, PDB-6p65: HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antigen binding 手法: EM (単粒子) / 解像度: 3.94 Å EMDB-20273: Negative-stain reconstruction of HIV-1 Env BG505 NFL CC+ trimer in complex with rabbit antibody E70 fragment antigen binding 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-20274: Negative stain reconstruction of HIV-1 Env 16055 NFL TD 2CC+ trimer in complex with rabbit antibody 1C2 fragment antigen binding 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-20279: Polyclonal Fabs from rabbit C3 post 5th immunization in complex with heterologous Env trimer 45_01dH5 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-20280: Polyclonal Fabs from rabbit C3 post 5th immunization in complex with heterologous Env trimer 45_01dH5 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-20342: Polyclonal serum of rabbit C3 boost 5 in complex with heterologous Env trimer SC422 NFL TD CC+ 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-20343: Polyclonal serum from rabbit C3 post 4th immunization in complex with heterologous Env trimer 45_01dG5 NFL TD 2CC+ 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-20344: Polyclonal serum of rabbit C3 post 5th immunization in complex with heterologous Env trimer 45_01dG5 NFL TD 2CC+ 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-20345: Polyclonal serum of rabbit A1 post 6th immunization in complex with homologous Env trimer 16055 NFL TD CC+ 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-20346: Polyclonal serum of rabbit A1 post 6th immunization in complex with heterologous Env trimer 45_01dG5 NFL TD 2CC+, class 1 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-20347: Polyclonal serum of rabbit A1 post 6th immunization in complex with heterologous Env trimer 45_01dG5 NFL TD 2CC+, class 2 手法: EM (単粒子) / 解像度: 24.0 Å PDB-6peh: Crystal structure of rabbit monoclonal anti-HIV antibody 1C2 手法: X-RAY DIFFRACTION / 解像度: 2.296 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-HOH: WATER / 水
由来	human immunodeficiency virus 1 (ヒト免疫不全ウイルス) oryctolagus cuniculus (ウサギ) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/immune system (ウイルス性) / HIV-1 / CD4 binding site / neutralizing antibody (中和抗体) / rabbit antibody / VIRAL PROTEIN (ウイルスタンパク質) / VIRAL PROTEIN-immune system complex (ウイルス性) / IMMUNE SYSTEM (免疫系) / Antibody (抗体) / bnAb / HIV (ヒト免疫不全ウイルス)