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-Structure paper
タイトル | High-Resolution Structure and Internal Mobility of a Plant 40S Ribosomal Subunit. |
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ジャーナル・号・ページ | Int J Mol Sci, Vol. 24, Issue 24, Year 2023 |
掲載日 | 2023年12月14日 |
著者 | Olesya V Kravchenko / Timur N Baymukhametov / Zhanna A Afonina / Konstantin S Vassilenko / |
PubMed 要旨 | Ribosome is a major part of the protein synthesis machinery, and analysis of its structure is of paramount importance. However, the structure of ribosomes from only a limited number of organisms has ...Ribosome is a major part of the protein synthesis machinery, and analysis of its structure is of paramount importance. However, the structure of ribosomes from only a limited number of organisms has been resolved to date; it especially concerns plant ribosomes and ribosomal subunits. Here, we report a high-resolution cryo-electron microscopy reconstruction of the small subunit of the (common wheat) cytoplasmic ribosome. A detailed atomic model was built that includes the majority of the rRNA and some of the protein modifications. The analysis of the obtained data revealed structural peculiarities of the 40S subunit in the monocot plant ribosome. We applied the 3D Flexible Refinement approach to analyze the internal mobility of the 40S subunit and succeeded in decomposing it into four major motions, describing rotations of the head domain and a shift in the massive rRNA expansion segment. It was shown that these motions are almost uncorrelated and that the 40S subunit is flexible enough to spontaneously adopt any conformation it takes as a part of a translating ribosome or ribosomal complex. Here, we introduce the first high-resolution structure of an isolated plant 40S subunit and the first quantitative analysis of the flexibility of small ribosomal subunits, hoping that it will help in studying various aspects of ribosome functioning. |
リンク | Int J Mol Sci / PubMed:38139282 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.34 - 2.68 Å |
構造データ | EMDB-18903, PDB-8r57: EMDB-18911: 3DFlex refinement of wheat 40S ribosomal subunit cryo-EM reconstruction EMDB-18951, PDB-8r6f: |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-K: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / small ribosomal subunit / wheat / eukaryotes / 40S |