EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of wild-type and a constitutively closed mutant of connexin26 shed light on channel regulation by CO.
ジャーナル・号・ページ	Elife, Vol. 13, Year 2024
掲載日	2024年6月3日
著者	Deborah H Brotherton / Sarbjit Nijjar / Christos G Savva / Nicholas Dale / Alexander David Cameron /
PubMed 要旨	Connexins allow intercellular communication by forming gap junction channels (GJCs) between juxtaposed cells. Connexin26 (Cx26) can be regulated directly by CO. This is proposed to be mediated ...Connexins allow intercellular communication by forming gap junction channels (GJCs) between juxtaposed cells. Connexin26 (Cx26) can be regulated directly by CO. This is proposed to be mediated through carbamylation of K125. We show that mutating K125 to glutamate, mimicking the negative charge of carbamylation, causes Cx26 GJCs to be constitutively closed. Through cryo-EM we observe that the K125E mutation pushes a conformational equilibrium towards the channel having a constricted pore entrance, similar to effects seen on raising the partial pressure of CO. In previous structures of connexins, the cytoplasmic loop, important in regulation and where K125 is located, is disordered. Through further cryo-EM studies we trap distinct states of Cx26 and observe density for the cytoplasmic loop. The interplay between the position of this loop, the conformations of the transmembrane helices and the position of the N-terminal helix, which controls the aperture to the pore, provides a mechanism for regulation.
リンク	Elife / PubMed:38829031 / PubMed Central
手法	EM (単粒子)
解像度	2.1 - 4.9 Å
構造データ	18290 8q9z EMDB-18290, PDB-8q9z: Cryo-EM structure of Cx26 gap junction K125E mutant in bicarbonate buffer (classification on hemichannel) 手法: EM (単粒子) / 解像度: 2.4 Å 18291 8qa0 EMDB-18291, PDB-8qa0: Cryo-EM structure of Cx26 solubilised in LMNG - hemichannel classification - NConst conformation 手法: EM (単粒子) / 解像度: 2.3 Å 18292 8qa1 EMDB-18292, PDB-8qa1: Cryo-EM structure of Cx26 solubilised in LMNG - Hemichannel classification NFlex conformation 手法: EM (単粒子) / 解像度: 2.3 Å 18293 8qa2 EMDB-18293, PDB-8qa2: Cryo-EM structure of Cx26 solubilised in LMNG: classification on subunit A; Nconst-mon conformation 手法: EM (単粒子) / 解像度: 2.3 Å 18294 8qa3 EMDB-18294, PDB-8qa3: Cryo-EM structure of Cx26 solubilised in LMNG: classification on subunit A; NFlex conformation 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-18295: Cryo-EM reconstruction of Cx26 gap junction K125R mutant (D6 symmetry) 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-18296: Cryo-EM reconstruction of Cx26 gap junction K125E mutant in HEPES buffer 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-18297: Cryo-EM reconstruction of Cx26 gap junction WT in HEPES buffer 手法: EM (単粒子) / 解像度: 4.9 Å
化合物	ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-HOH: WATER
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / Gap junction Large Pore Channel Carbon dioxide sensitive