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-Structure paper
| タイトル | Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 4783, Year 2024 |
| 掲載日 | 2024年6月5日 |
 著者 | Iskander Khusainov / Natalie Romanov / Camille Goemans / Beata Turoňová / Christian E Zimmerli / Sonja Welsch / Julian D Langer / Athanasios Typas / Martin Beck /    |
| PubMed 要旨 | Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal ...Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative bacteria are lacking, despite their ribosomes being major antimicrobial drug targets. Here we compare two E. coli strains, a lab E. coli K-12 and human gut isolate E. coli ED1a, for which tetracycline exhibits bacteriostatic and bactericidal action, respectively. Using our approach for close-to-native E. coli sample preparation, we assess the two strains by cryo-ET and visualize their ribosomes at high resolution in situ. Upon tetracycline treatment, these exhibit virtually identical drug binding sites, yet the conformation distribution of ribosomal complexes differs. While K-12 retains ribosomes in a translation-competent state, tRNAs are lost in the vast majority of ED1a ribosomes. These structural findings together with the proteome-wide abundance and thermal stability assessments indicate that antibiotic responses are complex in cells and can differ between different strains of a single species, thus arguing that all relevant bacterial strains should be analyzed in situ when addressing antibiotic mode of action. |
 リンク | Nat Commun / PubMed:38839776 / PubMed Central |
| 手法 | EM (サブトモグラム平均) / EM (単粒子) |
| 解像度 | 2.75 - 8.5 Å |
| 構造データ |  EMDB-18036: In situ structure of E. coli 70S ribosome  EMDB-18037: In situ 70S ribosome of E. coli K-12 untreated cells  EMDB-18038: In situ 70S ribosome of E. coli K-12 cells treated with tetracycline  EMDB-18039: In situ 70S ribosome of E. coli ED1a untreated cells  EMDB-18040: In situ 70S ribosome of E. coli ED1a cells treated with tetracycline  EMDB-18041: E. coli K-12 70S ribosome bound to mRNA A-tRNA, P-tRNA, E-tRNA  EMDB-18042: E. coli ED1a 70S ribosome bound to mRNA A-tRNA, P-tRNA, E-tRNA  EMDB-19206: E. coli ED1a 70S-tetracycline complex - focused refinement on 30S head  EMDB-19207: E. coli ED1a 70S-tetracycline complex - focused refinement on 30S body  EMDB-19208: E. coli ED1a 70S-tetracycline complex - focused refinement on 50S |
| 由来 |
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Escherichia coli BW25113 (大腸菌)