EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	CryoEM insights into RNA primer synthesis by the human primosome.
ジャーナル・号・ページ	FEBS J, Vol. 291, Issue 8, Page 1813-1829, Year 2024
掲載日	2024年2月9日
著者	Zhan Yin / Mairi L Kilkenny / De-Sheng Ker / Luca Pellegrini /
PubMed 要旨	Eukaryotic DNA replication depends on the primosome - a complex of DNA polymerase alpha (Pol α) and primase - to initiate DNA synthesis by polymerisation of an RNA-DNA primer. Primer synthesis ...Eukaryotic DNA replication depends on the primosome - a complex of DNA polymerase alpha (Pol α) and primase - to initiate DNA synthesis by polymerisation of an RNA-DNA primer. Primer synthesis requires the tight coordination of primase and polymerase activities. Recent cryo-electron microscopy (cryoEM) analyses have elucidated the extensive conformational transitions required for RNA primer handover between primase and Pol α and primer elongation by Pol α. Because of the intrinsic flexibility of the primosome, however, structural information about the initiation of RNA primer synthesis is still lacking. Here, we capture cryoEM snapshots of the priming reaction to reveal the conformational trajectory of the human primosome that brings DNA primase subunits 1 and 2 (PRIM1 and PRIM2, respectively) together, poised for RNA synthesis. Furthermore, we provide experimental evidence for the continuous association of primase subunit PRIM2 with the RNA primer during primer synthesis, and for how both initiation and termination of RNA primer polymerisation are licenced by specific rearrangements of DNA polymerase alpha catalytic subunit (POLA1), the polymerase subunit of Pol α. Our findings fill a critical gap in our understanding of the conformational changes that underpin the synthesis of the RNA primer by the primosome. Together with existing evidence, they provide a complete description of the structural dynamics of the human primosome during DNA replication initiation.
リンク	FEBS J / PubMed:38335062
手法	EM (単粒子)
解像度	3.07 - 6.15 Å
構造データ	17795 8qj7 EMDB-17795, PDB-8qj7: Cryo-EM structure of human DNA polymerase alpha-primase in pre-initiation stage 1 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-17807: Cryo-EM structure of human DNA polymerase alpha-primase in pre-initiation stage 2 手法: EM (単粒子) / 解像度: 4.11 Å EMDB-17810: Cryo-EM structure of human DNA polymerase alpha-primase in pre-initiation stage 3 手法: EM (単粒子) / 解像度: 3.97 Å EMDB-17811: Cryo-EM structure of human DNA polymerase alpha-primase in pre-initiation stage 4 手法: EM (単粒子) / 解像度: 4.21 Å EMDB-17812: Cryo-EM structure of human DNA polymerase alpha-primase at initiation I 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-17813: Cryo-EM structure of human DNA polymerase alpha-primase at initiation II 手法: EM (単粒子) / 解像度: 4.03 Å EMDB-17824: Cryo-EM structure of human DNA polymerase alpha-primase in primer handover 手法: EM (単粒子) / 解像度: 6.15 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-SF4*: IRON/SULFUR CLUSTER
由来	homo sapiens (ヒト)
キーワード	DNA BINDING PROTEIN / DNA polymerase / complex