EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 1756, Year 2024
掲載日	2024年2月26日
著者	Timo Flügel / Magdalena Schacherl / Anett Unbehaun / Birgit Schroeer / Marylena Dabrowski / Jörg Bürger / Thorsten Mielke / Thiemo Sprink / Christoph A Diebolder / Yollete V Guillén Schlippe / Christian M T Spahn /
PubMed 要旨	Structural studies of translating ribosomes traditionally rely on in vitro assembly and stalling of ribosomes in defined states. To comprehensively visualize bacterial translation, we reactivated ex ...Structural studies of translating ribosomes traditionally rely on in vitro assembly and stalling of ribosomes in defined states. To comprehensively visualize bacterial translation, we reactivated ex vivo-derived E. coli polysomes in the PURE in vitro translation system and analyzed the actively elongating polysomes by cryo-EM. We find that 31% of 70S ribosomes assemble into disome complexes that represent eight distinct functional states including decoding and termination intermediates, and a pre-nucleophilic attack state. The functional diversity of disome complexes together with RNase digest experiments suggests that paused disome complexes transiently form during ongoing elongation. Structural analysis revealed five disome interfaces between leading and queueing ribosomes that undergo rearrangements as the leading ribosome traverses through the elongation cycle. Our findings reveal at the molecular level how bL9's CTD obstructs the factor binding site of queueing ribosomes to thwart harmful collisions and illustrate how translation dynamics reshape inter-ribosomal contacts.
リンク	Nat Commun / PubMed:38409277 / PubMed Central
手法	EM (単粒子)
解像度	3.09 - 9.11 Å
構造データ	EMDB-17274: Escherichia coli paused disome complex (decoding \| rotated PRE) 手法: EM (単粒子) / 解像度: 9.06 Å EMDB-17275: Escherichia coli paused disome complex (decoding \| closed non-rot. PRE) 手法: EM (単粒子) / 解像度: 8.18 Å EMDB-17276: Escherichia coli paused disome complex (decoding \| POST) 手法: EM (単粒子) / 解像度: 9.11 Å EMDB-17277: Escherichia coli paused disome complex (partially accommodated A-tRNA \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 7.9 Å EMDB-17278: Escherichia coli paused disome complex (partially accommodated A-tRNA \| rotated PRE) 手法: EM (単粒子) / 解像度: 8.93 Å EMDB-17279: Escherichia coli paused disome complex (open non-rotated PRE \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 8.41 Å EMDB-17280: Escherichia coli paused disome complex (open non-rotated PRE \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 6.95 Å EMDB-17281: Escherichia coli paused disome complex (open non-rotated PRE \| rotated PRE) 手法: EM (単粒子) / 解像度: 7.54 Å EMDB-17282: Escherichia coli paused disome complex (open non-rotated PRE \| POST) 手法: EM (単粒子) / 解像度: 7.07 Å EMDB-17283: Escherichia coli paused disome complex (closed non-rotated PRE \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 4.95 Å EMDB-17284: Escherichia coli paused disome complex (closed non-rotated PRE \| rotated PRE) 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-17285: Escherichia coli paused disome complex (closed non-rotated PRE \| POST) 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-17286: Escherichia coli paused disome complex (rotated-PRE-1 \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 8.19 Å EMDB-17288: Escherichia coli paused disome complex (rotated-PRE-1 \| rotated PRE) 手法: EM (単粒子) / 解像度: 8.62 Å EMDB-17289: Cryo-EM reconstruction of the E. coli disome complex (rotated-PRE-1 \| POST) 手法: EM (単粒子) / 解像度: 8.9 Å EMDB-17291: Escherichia coli paused disome complex (rotated-PRE-2 \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 7.89 Å EMDB-17431: Escherichia coli paused disome complex (rotated-PRE-2 \| rotated PRE) 手法: EM (単粒子) / 解像度: 7.58 Å EMDB-17432: Escherichia coli paused disome complex (rotated-PRE-2 \| POST) 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-17433: Escherichia coli paused disome complex (POST \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-17434: Escherichia coli paused disome complex (POST \| rotated PRE) 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-17441: Escherichia coli paused disome complex (POST \| POST) 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-17442: Escherichia coli paused disome complex (termination \| closed non-rotated PRE) 手法: EM (単粒子) / 解像度: 6.71 Å EMDB-17443: Escherichia coli paused disome complex (termination \| rotated PRE) 手法: EM (単粒子) / 解像度: 7.03 Å EMDB-17444: Escherichia coli paused disome complex (termination \| POST) 手法: EM (単粒子) / 解像度: 6.66 Å 17631 8peg EMDB-17631, PDB-8peg: Escherichia coli paused disome complex (queueing 70S non-rotated closed PRE state) 手法: EM (単粒子) / 解像度: 3.3 Å 17743 8pkl EMDB-17743, PDB-8pkl: Escherichia coli paused disome complex (leading 70S non-rotated closed PRE state) 手法: EM (単粒子) / 解像度: 3.09 Å 18875 8r3v EMDB-18875, PDB-8r3v: Escherichia coli paused disome complex (non-rotated disome interface) 手法: EM (単粒子) / 解像度: 3.28 Å 19054 8rcl EMDB-19054, PDB-8rcl: Escherichia coli paused disome complex (Non-rotated disome interface class 1) 手法: EM (単粒子) / 解像度: 3.49 Å 19055 8rcm EMDB-19055, PDB-8rcm: Escherichia coli paused disome complex (Non-rotated disome interface class 2) 手法: EM (単粒子) / 解像度: 3.59 Å 19058 8rcs EMDB-19058, PDB-8rcs: Escherichia coli paused disome complex (Rotated disome interface class 1) 手法: EM (単粒子) / 解像度: 4.46 Å 19059 8rct EMDB-19059, PDB-8rct: Escherichia coli paused disome complex (Rotated disome interface class 2) 手法: EM (単粒子) / 解像度: 5.32 Å EMDB-19094: Escherichia coli paused disome complex (local refinement of leading decoding 70S) 手法: EM (単粒子) / 解像度: 4.61 Å EMDB-19095: Escherichia coli paused disome complex (local refinement of leading 70S partially accommodated A-tRNA) 手法: EM (単粒子) / 解像度: 4.56 Å EMDB-19096: Escherichia coli paused disome complex (local refinement of leading 70S rotated PRE-1) 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-19097: Escherichia coli paused disome complex (local refinement of leading 70S rotated PRE-2) 手法: EM (単粒子) / 解像度: 3.22 Å EMDB-19098: Escherichia coli paused disome complex (local refinement of leading 70S open non-rotated PRE) 手法: EM (単粒子) / 解像度: 3.79 Å EMDB-19099: Escherichia coli paused disome complex (local refinement of leading 70S termination) 手法: EM (単粒子) / 解像度: 3.82 Å EMDB-19100: Escherichia coli paused disome complex (local refinement of queueing 70S rotated PRE-2) 手法: EM (単粒子) / 解像度: 3.21 Å EMDB-19101: Escherichia coli paused trisome complex (non-rotated) 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-19102: Escherichia coli paused trisome complex (rotated) 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-19103: Escherichia coli paused disome complex (local refinement of queueing 70S POST) 手法: EM (単粒子) / 解像度: 3.21 Å EMDB-19104: Escherichia coli paused disome complex (local refinement of leading 70S POST) 手法: EM (単粒子) / 解像度: 3.21 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-PUT: 1,4-DIAMINOBUTANE / プトレシン ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-ALA: ALANINE / アラニン ChemComp-HOH*: WATER
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME / translation / disome / collision / PURE system / polysome / tranlsation / elongation / pausing