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-Structure paper
タイトル | Structural basis of ubiquitin-independent PP1 complex disassembly by p97. |
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ジャーナル・号・ページ | EMBO J, Vol. 42, Issue 14, Page e113110, Year 2023 |
掲載日 | 2023年7月17日 |
著者 | Johannes van den Boom / Guendalina Marini / Hemmo Meyer / Helen R Saibil / |
PubMed 要旨 | The AAA+-ATPase p97 (also called VCP or Cdc48) unfolds proteins and disassembles protein complexes in numerous cellular processes, but how substrate complexes are loaded onto p97 and disassembled is ...The AAA+-ATPase p97 (also called VCP or Cdc48) unfolds proteins and disassembles protein complexes in numerous cellular processes, but how substrate complexes are loaded onto p97 and disassembled is unclear. Here, we present cryo-EM structures of p97 in the process of disassembling a protein phosphatase-1 (PP1) complex by extracting an inhibitory subunit from PP1. We show that PP1 and its partners SDS22 and inhibitor-3 (I3) are loaded tightly onto p97, surprisingly via a direct contact of SDS22 with the p97 N-domain. Loading is assisted by the p37 adapter that bridges two adjacent p97 N-domains underneath the substrate complex. A stretch of I3 is threaded into the central channel of the spiral-shaped p97 hexamer, while other elements of I3 are still attached to PP1. Thus, our data show how p97 arranges a protein complex between the p97 N-domain and central channel, suggesting a hold-and-extract mechanism for p97-mediated disassembly. |
リンク | EMBO J / PubMed:37264685 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.32 - 8.2 Å |
構造データ | EMDB-15774: p97 with I3 substrate in channel EMDB-15778: p97-substrate complex with SPI substrate on subunit D EMDB-15846: p97-SPI substrate complex on subunit E EMDB-15847: p97 complexed with SPI substrate on subunit C EMDB-15861, PDB-8b5r: |
由来 |
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キーワード | CHAPERONE / AAA+ ATPase / p97 / VCP / Cdc48 / unfoldase / protein phosphatase-1 / protein maturation |