EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural evolution of fibril polymorphs during amyloid assembly.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 26, Page 5798-5811.e26, Year 2023
掲載日	2023年12月21日
著者	Martin Wilkinson / Yong Xu / Dev Thacker / Alexander I P Taylor / Declan G Fisher / Rodrigo U Gallardo / Sheena E Radford / Neil A Ranson /
PubMed 要旨	Cryoelectron microscopy (cryo-EM) has provided unprecedented insights into amyloid fibril structures, including those associated with disease. However, these structures represent the endpoints of ...Cryoelectron microscopy (cryo-EM) has provided unprecedented insights into amyloid fibril structures, including those associated with disease. However, these structures represent the endpoints of long assembly processes, and their relationship to fibrils formed early in assembly is unknown. Consequently, whether different fibril architectures, with potentially different pathological properties, form during assembly remains unknown. Here, we used cryo-EM to determine structures of amyloid fibrils at different times during in vitro fibrillation of a disease-related variant of human islet amyloid polypeptide (IAPP-S20G). Strikingly, the fibrils formed in the lag, growth, and plateau phases have different structures, with new forms appearing and others disappearing as fibrillation proceeds. A time course with wild-type hIAPP also shows fibrils changing with time, suggesting that this is a general property of IAPP amyloid assembly. The observation of transiently populated fibril structures has implications for understanding amyloid assembly mechanisms with potential new insights into amyloid progression in disease.
リンク	Cell / PubMed:38134875
手法	EM (らせん対称)
解像度	2.2 - 3.4 Å
構造データ	15696 8awt EMDB-15696, PDB-8awt: IAPP S20G lag-phase fibril polymorph 2PF-P 手法: EM (らせん対称) / 解像度: 3.0 Å 15728 8az0 EMDB-15728, PDB-8az0: IAPP S20G growth-phase fibril polymorph 2PF-L 手法: EM (らせん対称) / 解像度: 3.4 Å 15729 8az1 EMDB-15729, PDB-8az1: IAPP S20G growth-phase fibril polymorph 2PF-C 手法: EM (らせん対称) / 解像度: 3.1 Å 15730 8az2 EMDB-15730, PDB-8az2: IAPP S20G growth-phase fibril polymorph 3PF-CU 手法: EM (らせん対称) / 解像度: 3.4 Å 15731 8az3 EMDB-15731, PDB-8az3: IAPP S20G growth-phase fibril polymorph 4PF-CU 手法: EM (らせん対称) / 解像度: 3.4 Å 15753 8az4 EMDB-15753, PDB-8az4: IAPP S20G plateau-phase fibril polymorph 2PF-L 手法: EM (らせん対称) / 解像度: 2.2 Å 15754 8az5 EMDB-15754, PDB-8az5: IAPP S20G plateau-phase fibril polymorph 4PF-CU 手法: EM (らせん対称) / 解像度: 2.3 Å 15755 8az6 EMDB-15755, PDB-8az6: IAPP S20G plateau-phase fibril polymorph 4PF-LU 手法: EM (らせん対称) / 解像度: 3.1 Å 15756 8az7 EMDB-15756, PDB-8az7: IAPP S20G plateau-phase fibril polymorph 4PF-LJ 手法: EM (らせん対称) / 解像度: 2.9 Å
化合物	ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト)
キーワード	PROTEIN FIBRIL / Amyloid (アミロイド) / fibril (フィブリル) / helical / cross-beta / amylin (アミリン (ホルモン)) / polymorph / islet / Diabetes (糖尿病)