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-Structure paper
タイトル | A succession of two viral lattices drives vaccinia virus assembly. |
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ジャーナル・号・ページ | PLoS Biol, Vol. 21, Issue 3, Page e3002005, Year 2023 |
掲載日 | 2023年3月2日 |
![]() | Miguel Hernandez-Gonzalez / Thomas Calcraft / Andrea Nans / Peter B Rosenthal / Michael Way / ![]() |
PubMed 要旨 | During its cytoplasmic replication, vaccinia virus assembles non-infectious spherical immature virions (IV) coated by a viral D13 lattice. Subsequently, IV mature into infectious brick-shaped ...During its cytoplasmic replication, vaccinia virus assembles non-infectious spherical immature virions (IV) coated by a viral D13 lattice. Subsequently, IV mature into infectious brick-shaped intracellular mature virions (IMV) that lack D13. Here, we performed cryo-electron tomography (cryo-ET) of frozen-hydrated vaccinia-infected cells to structurally characterise the maturation process in situ. During IMV formation, a new viral core forms inside IV with a wall consisting of trimeric pillars arranged in a new pseudohexagonal lattice. This lattice appears as a palisade in cross-section. As maturation occurs, which involves a 50% reduction in particle volume, the viral membrane becomes corrugated as it adapts to the newly formed viral core in a process that does not appear to require membrane removal. Our study suggests that the length of this core is determined by the D13 lattice and that the consecutive D13 and palisade lattices control virion shape and dimensions during vaccinia assembly and maturation. |
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手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 19.2 - 30.6 Å |
構造データ | ![]() EMDB-15596: In situ subtomogram average of Vaccinia virus (WR) palisade, all virions ![]() EMDB-15597: In situ subtomogram average of Vaccinia virus (WR) palisade, from CEVs ![]() EMDB-15598: In situ subtomogram average of Vaccinia virus (WR) palisade, from IMVs ![]() EMDB-15599: In situ subtomogram average of Vaccinia virus (WR) palisade, from IEVs ![]() EMDB-15600: Cryotomogram of Vaccinia virus (WR) infected HeLa cell (immature virions) ![]() EMDB-15601: Cryotomogram of Vaccinia virus (WR) infected HeLa cell (mature virions) EMDB-15602, PDB-8arh: |
由来 |
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![]() | VIRUS / Palisade / Lattice / A4 |