+検索条件
-Structure paper
タイトル | Structural insights into RNA-mediated transcription regulation in bacteria. |
---|---|
ジャーナル・号・ページ | Mol Cell, Vol. 82, Issue 20, Page 3885-3900.e10, Year 2022 |
掲載日 | 2022年10月20日 |
著者 | Sanjay Dey / Claire Batisse / Jinal Shukla / Michael W Webster / Maria Takacs / Charlotte Saint-André / Albert Weixlbaumer / |
PubMed 要旨 | RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at ...RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at intrinsic terminators in all kingdoms of life. In contrast, the regulatory RNA putL suppresses pausing and termination in cis. However, how nascent RNA modulates its own synthesis remains largely unknown. We present cryo-EM reconstructions of RNAP captured during transcription of putL variants or an unrelated sequence at a U-rich pause site. Our results suggest how putL suppresses pausing and promotes its synthesis. We demonstrate that transcribing a U-rich sequence, a ubiquitous trigger of intrinsic termination, promotes widening of the RNAP nucleic-acid-binding channel. Widening destabilizes RNAP interactions with DNA and RNA to facilitate transcript dissociation reminiscent of intrinsic transcription termination. Surprisingly, RNAP remains bound to DNA after transcript release. Our results provide the structural framework to understand RNA-mediated intrinsic transcription termination. |
リンク | Mol Cell / PubMed:36220101 |
手法 | EM (単粒子) |
解像度 | 3.4 - 4.5 Å |
構造データ | EMDB-15327, PDB-8aby: EMDB-15328, PDB-8abz: EMDB-15329, PDB-8ac0: EMDB-15330, PDB-8ac1: EMDB-15331, PDB-8ac2: EMDB-15352, PDB-8acp: EMDB-15357, PDB-8ad1: EMDB-15612: RNA polymerase at U-rich pause bound to regulatory RNA putL - Pause-prone, closed clamp state EMDB-15613: RNA polymerase bound to purified in vitro transcribed regulatory RNA putL - inactive, open clamp state |
化合物 | ChemComp-MG: ChemComp-ZN: |
由来 |
|
キーワード | TRANSCRIPTION / RNA polymerase / transcriptional pausing / transcription termination / regulatory RNA |