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-Structure paper
タイトル | Insights into complex I assembly: Function of NDUFAF1 and a link with cardiolipin remodeling. |
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ジャーナル・号・ページ | Sci Adv, Vol. 8, Issue 46, Page eadd3855, Year 2022 |
掲載日 | 2022年11月18日 |
著者 | Jonathan Schiller / Eike Laube / Ilka Wittig / Werner Kühlbrandt / Janet Vonck / Volker Zickermann / |
PubMed 要旨 | Respiratory complex I is a ~1-MDa proton pump in mitochondria. Its structure has been revealed in great detail, but the structural basis of its assembly, in humans involving at least 15 assembly ...Respiratory complex I is a ~1-MDa proton pump in mitochondria. Its structure has been revealed in great detail, but the structural basis of its assembly, in humans involving at least 15 assembly factors, is essentially unknown. We determined cryo-electron microscopy structures of assembly intermediates associated with assembly factor NDUFAF1 in a yeast model system. Subunits ND2 and NDUFC2 together with assembly factors NDUFAF1 and CIA84 form the nucleation point of the NDUFAF1-dependent assembly pathway. Unexpectedly, the cardiolipin remodeling enzyme tafazzin is an integral component of this core complex. In a later intermediate, all 12 subunits of the proximal proton pump module have assembled. NDUFAF1 locks the central ND3 subunit in an assembly-competent conformation, and major rearrangements of central subunits are required for complex I maturation. |
リンク | Sci Adv / PubMed:36383672 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.9 - 3.2 Å |
構造データ | EMDB-14764, PDB-7zkp: EMDB-14765, PDB-7zkq: EMDB-14766: Late Pp module assembly intermediate of complex I without assembly factors EMDB-14770: Late assembly intermediate of the proximal proton pumping module of complex I with assembly factor NDUFAF1 |
化合物 | ChemComp-CDL: ChemComp-PLC: ChemComp-CPL: ChemComp-3PE: ChemComp-T7X: ChemComp-LMN: |
由来 |
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キーワード | ELECTRON TRANSPORT / assembly respiratory chain membrane protein mitochondria |