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-Structure paper
タイトル | Structural basis for safe and efficient energy conversion in a respiratory supercomplex. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 545, Year 2022 |
掲載日 | 2022年1月27日 |
著者 | Wei-Chun Kao / Claire Ortmann de Percin Northumberland / Tat Cheung Cheng / Julio Ortiz / Alexandre Durand / Ottilie von Loeffelholz / Oliver Schilling / Martin L Biniossek / Bruno P Klaholz / Carola Hunte / |
PubMed 要旨 | Proton-translocating respiratory complexes assemble into supercomplexes that are proposed to increase the efficiency of energy conversion and limit the production of harmful reactive oxygen species ...Proton-translocating respiratory complexes assemble into supercomplexes that are proposed to increase the efficiency of energy conversion and limit the production of harmful reactive oxygen species during aerobic cellular respiration. Cytochrome bc complexes and cytochrome aa oxidases are major drivers of the proton motive force that fuels ATP generation via respiration, but how wasteful electron- and proton transfer is controlled to enhance safety and efficiency in the context of supercomplexes is not known. Here, we address this question with the 2.8 Å resolution cryo-EM structure of the cytochrome bcc-aa (III-IV) supercomplex from the actinobacterium Corynebacterium glutamicum. Menaquinone, substrate mimics, lycopene, an unexpected Q site, dioxygen, proton transfer routes, and conformational states of key protonable residues are resolved. Our results show how safe and efficient energy conversion is achieved in a respiratory supercomplex through controlled electron and proton transfer. The structure may guide the rational design of drugs against actinobacteria that cause diphtheria and tuberculosis. |
リンク | Nat Commun / PubMed:35087070 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 - 3.1 Å |
構造データ | EMDB-13976, PDB-7qhm: EMDB-13977, PDB-7qho: |
化合物 | ChemComp-FES: ChemComp-IZL: ChemComp-9YF: ChemComp-MQ9: ChemComp-3PE: ChemComp-SMA: ChemComp-HEM: ChemComp-CDL: ChemComp-LYC: ChemComp-LMT: ChemComp-HEC: ChemComp-HAS: ChemComp-CU: ChemComp-MN: ChemComp-CA: ChemComp-AZI: ChemComp-CUA: ChemComp-DGA: ChemComp-PLM: ChemComp-IX7: ChemComp-OXY: ChemComp-HOH: ChemComp-3PH: |
由来 |
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キーワード | OXIDOREDUCTASE / supercomplex / respiratory chain / cytochrome bcc-aa3 supercomplex / proton translocation / bioenergetics / membrane protein / electron transport |