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- PDB-7qho: Cytochrome bcc-aa3 supercomplex (respiratory supercomplex III2/IV... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7qho | ||||||||||||||||||
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Title | Cytochrome bcc-aa3 supercomplex (respiratory supercomplex III2/IV2) from Corynebacterium glutamicum (as isolated) | ||||||||||||||||||
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![]() | OXIDOREDUCTASE / supercomplex / respiratory chain / cytochrome bcc-aa3 supercomplex / proton translocation / bioenergetics / membrane protein / electron transport | ||||||||||||||||||
Function / homology | ![]() thiamine biosynthetic process / aerobic electron transport chain / cytochrome-c oxidase / oxidative phosphorylation / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / : / electron transport coupled proton transport / ATP synthesis coupled electron transport ...thiamine biosynthetic process / aerobic electron transport chain / cytochrome-c oxidase / oxidative phosphorylation / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / : / electron transport coupled proton transport / ATP synthesis coupled electron transport / respiratory electron transport chain / electron transport chain / 2 iron, 2 sulfur cluster binding / membrane => GO:0016020 / oxidoreductase activity / iron ion binding / copper ion binding / heme binding / membrane / metal ion binding / plasma membrane Similarity search - Function | ||||||||||||||||||
Biological species | ![]() | ||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å | ||||||||||||||||||
![]() | Kao, W.-C. / Hunte, C. | ||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Structural basis for safe and efficient energy conversion in a respiratory supercomplex. Authors: Wei-Chun Kao / Claire Ortmann de Percin Northumberland / Tat Cheung Cheng / Julio Ortiz / Alexandre Durand / Ottilie von Loeffelholz / Oliver Schilling / Martin L Biniossek / Bruno P Klaholz / Carola Hunte / ![]() ![]() Abstract: Proton-translocating respiratory complexes assemble into supercomplexes that are proposed to increase the efficiency of energy conversion and limit the production of harmful reactive oxygen species ...Proton-translocating respiratory complexes assemble into supercomplexes that are proposed to increase the efficiency of energy conversion and limit the production of harmful reactive oxygen species during aerobic cellular respiration. Cytochrome bc complexes and cytochrome aa oxidases are major drivers of the proton motive force that fuels ATP generation via respiration, but how wasteful electron- and proton transfer is controlled to enhance safety and efficiency in the context of supercomplexes is not known. Here, we address this question with the 2.8 Å resolution cryo-EM structure of the cytochrome bcc-aa (III-IV) supercomplex from the actinobacterium Corynebacterium glutamicum. Menaquinone, substrate mimics, lycopene, an unexpected Q site, dioxygen, proton transfer routes, and conformational states of key protonable residues are resolved. Our results show how safe and efficient energy conversion is achieved in a respiratory supercomplex through controlled electron and proton transfer. The structure may guide the rational design of drugs against actinobacteria that cause diphtheria and tuberculosis. | ||||||||||||||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 1.9 MB | Display | ![]() |
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PDB format | ![]() | 1.7 MB | Display | ![]() |
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-Validation report
Summary document | ![]() | 4.2 MB | Display | ![]() |
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Full document | ![]() | 4.3 MB | Display | |
Data in XML | ![]() | 148.8 KB | Display | |
Data in CIF | ![]() | 197.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 13977MC ![]() 7qhmC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Assembly
Deposited unit | ![]()
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Components
-Protein , 4 types, 8 molecules ANGTKXMZ
#1: Protein | Mass: 45232.207 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: qcrA, Cgl2190, cg2404 Production host: ![]() References: UniProt: Q79VE8 #7: Protein | Mass: 15557.285 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: ctaF, Cgl2194, cg2408 Production host: ![]() References: UniProt: Q8NNK3, cytochrome-c oxidase #11: Protein | Mass: 8373.271 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: Cgl0818 Production host: ![]() References: UniProt: Q8NS61 #13: Protein | Mass: 17152.379 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: thiX, Cgl2332, cg2561 Production host: ![]() References: UniProt: P42461 |
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-Cytochrome bc1 complex cytochrome ... , 2 types, 4 molecules BOCP
#2: Protein | Mass: 59863.699 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: qcrB, Cgl2189, cg2403 Production host: ![]() References: UniProt: Q79VE9, quinol-cytochrome-c reductase #3: Protein | Mass: 29898.850 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: qcrC, Cgl2191, cg2405 Production host: ![]() References: UniProt: Q8NNK5, quinol-cytochrome-c reductase |
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-Cytochrome c oxidase subunit ... , 3 types, 6 molecules DQERFS
#4: Protein | Mass: 66340.164 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: ctaD, Cgl2523, cg2780 Production host: ![]() References: UniProt: Q79VD7, cytochrome-c oxidase #5: Protein | Mass: 36811.105 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: ctaC, Cgl2195, cg2409 Production host: ![]() References: UniProt: Q8NNK2, cytochrome-c oxidase #6: Protein | Mass: 22457.217 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: ctaE, Cgl2192, cg2406 Production host: ![]() References: UniProt: Q9AEL8, cytochrome-c oxidase |
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-Uncharacterized ... , 2 types, 4 molecules HUIV
#8: Protein | Mass: 16954.213 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: Cgl2664, cg2949 Production host: ![]() References: UniProt: Q8NMB4 #9: Protein | Mass: 16385.588 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Cgl2017, cg2211 Production host: ![]() References: UniProt: Q8NP09 |
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-Hypothetical membrane ... , 2 types, 4 molecules JWLY
#10: Protein | Mass: 12144.160 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: Cgl0373 Production host: ![]() References: UniProt: Q8NTD4 #12: Protein | Mass: 6628.905 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: Cgl0673 Production host: ![]() References: UniProt: Q8NSJ8 |
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-Sugars , 1 types, 2 molecules ![](data/chem/img/LMT.gif)
#22: Sugar |
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-Non-polymers , 19 types, 86 molecules ![](data/chem/img/FES.gif)
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![](data/chem/img/LYC.gif)
![](data/chem/img/HEC.gif)
![](data/chem/img/3PH.gif)
![](data/chem/img/HAS.gif)
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![](data/chem/img/MN.gif)
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#14: Chemical | #15: Chemical | |
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