EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 2915, Year 2022
掲載日	2022年5月25日
著者	Almutasem Saleh / Yasunori Noguchi / Ricardo Aramayo / Marina E Ivanova / Kathryn M Stevens / Alex Montoya / S Sunidhi / Nicolas Lopez Carranza / Marcin J Skwark / Christian Speck /
PubMed 要旨	The controlled assembly of replication forks is critical for genome stability. The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at ...The controlled assembly of replication forks is critical for genome stability. The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. At present, it remains poorly understood how DDK docks onto the helicase and how the kinase targets distal Mcm subunits for phosphorylation. Using cryo-electron microscopy and biochemical analysis we discovered that an interaction between the HBRCT domain of Dbf4 with Mcm2 serves as an anchoring point, which supports binding of DDK across the MCM2-7 double-hexamer interface and phosphorylation of Mcm4 on the opposite hexamer. Moreover, a rotation of DDK along its anchoring point allows phosphorylation of Mcm2 and Mcm6. In summary, our work provides fundamental insights into DDK structure, control and selective activation of the MCM2-7 helicase during DNA replication. Importantly, these insights can be exploited for development of novel DDK inhibitors.
リンク	Nat Commun / PubMed:35614055 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 18.0 Å
構造データ	13619 7pt6 EMDB-13619: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III (composite map) PDB-7pt6: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III 手法: EM (単粒子) / 解像度: 3.2 Å 13620 7pt7 EMDB-13620: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I (composite map) PDB-7pt7: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-13621: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III (B1 map) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-13622: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III (B2 map) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-13623: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III (B3 map) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-13624: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III (3D auto-refined map) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-13629: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state II (composite map) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-13631: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state II (B1 map) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-13635: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state II (B2 map) 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-13640: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state II (3D auto-refined map) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-13644: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state I (3D auto-refined map) 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-13645: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I (B1 map) 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-13646: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I (B2 map) 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-13647: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I (3D auto-refined map) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-13648: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state (3D auto-refined map) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-13649: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state A (binned 3D auto-refined map) 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-13650: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state B (binned 3D auto-refined map) 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-13651: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state C (binned 3D auto-refined map) 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-13652: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state D (binned 3D auto-refined map) 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-13653: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state E (binned 3D auto-refined map) 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-13655: Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, swiveled state F (binned 3D auto-refined map) 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-13656: Structure of double-stranded DNA-bound MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATP (3D auto-refined map) 手法: EM (単粒子) / 解像度: 9.1 Å EMDB-13657: Structure of double-stranded DNA-bound MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATP (B1 map) 手法: EM (単粒子) / 解像度: 8.3 Å EMDB-13658: Structure of double-stranded DNA-bound MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATP (B2 map) 手法: EM (単粒子) / 解像度: 11.0 Å EMDB-13659: Structure of double-stranded DNA-bound MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATP (B3 map) 手法: EM (単粒子) / 解像度: 9.8 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) Saccharomyces cerevisiae (パン酵母) synthetic construct (人工物)
キーワード	REPLICATION / Helicase / Activation / Kinase / Phosphorylation