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-Structure paper
タイトル | Structure of photosystem II reveals conformational flexibility of stacked and unstacked supercomplexes. |
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ジャーナル・号・ページ | Elife, Vol. 12, Year 2023 |
掲載日 | 2023年2月17日 |
著者 | Ido Caspy / Maria Fadeeva / Yuval Mazor / Nathan Nelson / |
PubMed 要旨 | Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on ...Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on earth . Our knowledge on the mechanism of water photooxidation was greatly advanced by high-resolution structures of prokaryotic PSII . Here, we show high-resolution cryogenic electron microscopy (cryo-EM) structures of eukaryotic PSII from the green alga at two distinct conformations. The conformers are also present in stacked PSII, exhibiting flexibility that may be relevant to the grana formation in chloroplasts of the green lineage. CP29, one of PSII associated light-harvesting antennae, plays a major role in distinguishing the two conformations of the supercomplex. We also show that the stacked PSII dimer, a form suggested to support the organisation of thylakoid membranes , can appear in many different orientations providing a flexible stacking mechanism for the arrangement of grana stacks in thylakoids. Our findings provide a structural basis for the heterogenous nature of the eukaryotic PSII on multiple levels. |
リンク | Elife / PubMed:36799903 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.43 - 4.0 Å |
構造データ | EMDB-13429, PDB-7pi0: EMDB-13430, PDB-7pi5: EMDB-13444, PDB-7pin: EMDB-13455, PDB-7piw: EMDB-13548, PDB-7pnk: |
化合物 | ChemComp-OEX: ChemComp-FE2: ChemComp-CL: ChemComp-CLA: ChemComp-PHO: ChemComp-BCR: ChemComp-NA: ChemComp-3PH: ChemComp-C7Z: ChemComp-LMG: ChemComp-DGD: ChemComp-LHG: ChemComp-LMT: ChemComp-SQD: ChemComp-SPH: ChemComp-DGA: ChemComp-BCT: ChemComp-PL9: ChemComp-HEM: ChemComp-RRX: ChemComp-CHL: ChemComp-LUT: ChemComp-NEX: ChemComp-XAT: ChemComp-PTY: ChemComp-LPX: ChemComp-HOH: ChemComp-GOL: ChemComp-LMK: ChemComp-4RF: ChemComp-ERG: |
由来 |
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キーワード | PHOTOSYNTHESIS / green algae / photosystem II / thylakoid / oxygen evolving complex / cryo-EM / stacking |