EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of AAA+ ATPase-mediated RuvAB-Holliday junction branch migration.
ジャーナル・号・ページ	Nature, Vol. 609, Issue 7927, Page 630-639, Year 2022
掲載日	2022年8月24日
著者	Jiri Wald / Dirk Fahrenkamp / Nikolaus Goessweiner-Mohr / Wolfgang Lugmayr / Luciano Ciccarelli / Oliver Vesper / Thomas C Marlovits /
PubMed 要旨	The Holliday junction is a key intermediate formed during DNA recombination across all kingdoms of life. In bacteria, the Holliday junction is processed by two homo-hexameric AAA+ ATPase RuvB motors, ...The Holliday junction is a key intermediate formed during DNA recombination across all kingdoms of life. In bacteria, the Holliday junction is processed by two homo-hexameric AAA+ ATPase RuvB motors, which assemble together with the RuvA-Holliday junction complex to energize the strand-exchange reaction. Despite its importance for chromosome maintenance, the structure and mechanism by which this complex facilitates branch migration are unknown. Here, using time-resolved cryo-electron microscopy, we obtained structures of the ATP-hydrolysing RuvAB complex in seven distinct conformational states, captured during assembly and processing of a Holliday junction. Five structures together resolve the complete nucleotide cycle and reveal the spatiotemporal relationship between ATP hydrolysis, nucleotide exchange and context-specific conformational changes in RuvB. Coordinated motions in a converter formed by DNA-disengaged RuvB subunits stimulate hydrolysis and nucleotide exchange. Immobilization of the converter enables RuvB to convert the ATP-contained energy into a lever motion, which generates the pulling force driving the branch migration. We show that RuvB motors rotate together with the DNA substrate, which, together with a progressing nucleotide cycle, forms the mechanistic basis for DNA recombination by continuous branch migration. Together, our data decipher the molecular principles of homologous recombination by the RuvAB complex, elucidate discrete and sequential transition-state intermediates for chemo-mechanical coupling of hexameric AAA+ motors and provide a blueprint for the design of state-specific compounds targeting AAA+ motors.
リンク	Nature / PubMed:36002576 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 8.0 Å
構造データ	13294 7pbl EMDB-13294, PDB-7pbl: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s1 [t2 dataset] 手法: EM (単粒子) / 解像度: 3.2 Å 13295 7pbm EMDB-13295, PDB-7pbm: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s2 [t2 dataset] 手法: EM (単粒子) / 解像度: 3.2 Å 13296 7pbn EMDB-13296, PDB-7pbn: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s3 [t2 dataset] 手法: EM (単粒子) / 解像度: 3.2 Å 13297 7pbo EMDB-13297, PDB-7pbo: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s4 [t2 dataset] 手法: EM (単粒子) / 解像度: 2.9 Å 13298 7pbp EMDB-13298, PDB-7pbp: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s5 [t2 dataset] 手法: EM (単粒子) / 解像度: 3.2 Å 13299 7pbq EMDB-13299, PDB-7pbq: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s0+A [t2 dataset] 手法: EM (単粒子) / 解像度: 3.1 Å 13300 7pbr EMDB-13300, PDB-7pbr: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s0-A [t2 dataset] 手法: EM (単粒子) / 解像度: 3.0 Å 13301 7pbs EMDB-13301, PDB-7pbs: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s0+A [t1 dataset] 手法: EM (単粒子) / 解像度: 3.3 Å 13302 7pbt EMDB-13302, PDB-7pbt: RuvAB branch migration motor complexed to the Holliday junction - RuvB AAA+ state s1 [t1 dataset] 手法: EM (単粒子) / 解像度: 3.3 Å 13303 7pbu EMDB-13303, PDB-7pbu: RuvAB branch migration motor complexed to the Holliday junction - RuvA-HJ core [t2 dataset] 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-13304: RuvAB branch migration motor complexed to the Holliday junction - RuvAB-HJ half [dataset t2] 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-13305: RuvAB branch migration motor complexed to the Holliday junction - RuvAB-HJ pseudo half [dataset t2] 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-15085: RuvAB branch migration motor complexed to the Holliday junction - composite map 1 and 2 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-15126: RuvAB branch migration motor complexed to the Holliday junction - full tripartite particle [dataset t2] 手法: EM (単粒子) / 解像度: 8.0 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	streptococcus thermophilus (ストレプトコッカス・サリバリウス亜種サーモフィラス) salmonella typhimurium (サルモネラ菌) synthetic construct (人工物)
キーワード	HYDROLASE / DNA recombination / DNA repair / branch migration / Holliday junction / helicase